摘要
In order to establish a rapid propagation system for Brassica campestris L. ssp. chinensis var. utilis, the anther and cotyledon-cotyledonary petioles were used as explants to conduct tissue culture research. The results showed that not bloomed flower buds with higher styles than petals were appropriate for anther cul- ture. Moreover, most microspores were at the mid-late uninucleate stage at that time. The pollen germination rates of Brassica campestris L. ssp. chinensis var. u- tills were low and the germination rates in autumn and winter were higher than that in summer. The induction medium of anther callus of Brassica campestris L. ssp. chinensis var. utilis was MS+1.0 mg/L KT+1.0 mg/L 2, 4-D+3% sugar+6 g/L agar+ 8% coconut milk and the induction media of adventitious bud were MS+2.0 mg/L 6-BA+ 0.5 mg/L NAA+I.0 g/L activated carbon+2% sugar+6 g/L agar or MS+2.0 mg/L ZT+ 0.5 mg/L IAA+0.5 g/L AgNO3+1.0 g/L activated carbon+2% sugar+6 g/L agar. The induction percentage of adventitious bud by anther culture was 36.7%. Browning phenomenon appeared during the culture of adventitious bud and regeneration plant could not be formed. The plant regeneration rate reached 80% when cotyledon- cotyledonary petioles were used as explant.
为建立菜心(Brassica campestris ssp.chinensis var.utilis)的快繁技术体系,以花药和子叶-子叶柄为外植体进行组织培养研究。结果表明,花药培养以选取未开放的花蕾为宜,且花柱略高于花瓣,此时小孢子多数处于单核靠边期。菜心花粉的萌发率不高,且秋冬季的花粉比夏季的萌发率高。菜心花药愈伤组织诱导培养基为:MS+1.0 mg/L KT+1.0 mg/L 2,4-D+3%糖+6 g/L琼脂+8%椰乳,不定芽诱导培养基为:MS+2.0mg/L 6-BA+0.5 mg/L NAA+1.0 g/L活性炭+2%糖+6 g/L琼脂或MS+2.0 mg/L ZT+0.5 mg/L IAA+0.5 g/L Ag NO3+1.0g/L活性炭+2%糖+6 g/L琼脂。花药培养的不定芽诱导率为36.7%,不定芽培养出现褐化现象,不能形成再生植株;而以子叶-子叶柄为外植体培养获得的植株再生率可达80%。
基金
Supported by Guangdong Key Base Project of Scientific Research(2013A061401019)
Application Basis Project of Scientific and Informational Bureau in Guangzhou(2010Y1-C831)
Pearl River S&T Nova Program of Guangzhou(2013J2200086)~~