摘要
为了研究高羊茅14-3-3基因家族的功能,该研究以拟南芥已报道的13个14-3-3蛋白为基础,通过生物信息分析其潜在的功能,在前期高羊茅转录组与蛋白组学测序获得的14-3-3基因序列为基础,克隆获得4个高羊茅14-3-3基因。通过Clustal W2进行序列比对,发现14-3-3A与14-3-3D,14-3-3B与14-3-3C的同源关系比较近,并且主要结构十分保守。利用荧光定量PCR技术系统分析了14-3-3基因在低氮、干旱、高热以及高盐逆境胁迫下的表达变化发现14-3-3A基因能够应答低氮、干旱、高温以及高盐胁迫,其他成员也能部分应答上述胁迫,但应答机制不一样,可能是通过不同途径调节植物抗逆性,Fa14-3-3基因在进化过程中发生了功能分化。
To study the functions of 14-3-3 gene family in tall fescue, the potential functions of 13 14-3-3 proteins in Arabidopsis were investigated by bioinformatic analysis. Based on the sequences of 14-3-3 genes in tall fescue by transcriptome and proteomic sequencing, the full-length cDNA sequences of 4 14-3-3 genes in tall fescue were obtained. Their sequences were aligned by Clustal W2. The results showed that the genetic relationships between 14-3-3A and 14-3-3D, 14-3-3B and 14-3-3C are closer, and their main structures are very conservative. The changes in expression levels of 14-3-3 genes under low nitrogen, drought, high temperature and high salt stresses were investigated by fluorescence quantitative PCR. The expres- sion level of 14-3-3A makes responses to low nitrogen, drought, high temperature and high salt stresses; the expression levels of other genes also make responses to abiotic stresses in varying degrees, but the relevant response mechanisms are not exactly the same. Therefore, it is speculated that the 14-3-3 gene family regu- lates stress resistance of plants through different pathways, and functional differenti- ation occurs during its evolution.
基金
Supported by National Natural Science Foundation of China(31360576)~~
