预防性中药防治流感病毒作用及其免疫调节机制探讨

Antiviral Activity Against Influenza Virus and Immunoregulation Mechanism of Preventive Herbs

目的:研究预防性中药抗流感病毒及其黏膜免疫调节机制。方法:56只雌性SPF级BALB/C小鼠,随机分为造模组(48只)和正常组(8只),建立小鼠流感病毒H1N1/FM/1感染模型,造模成功后随机分为模型组、病毒唑组(0.1 g·kg-1),银翘散组(40 g·kg-1),玉屏风散组(60 g·kg-1),冰香散雾化组(0.6 g·kg-1),冰香散滴鼻组(0.03 g·kg-1),ig给药,5 d后处死小鼠,测定病毒载量,同时进行肺组织病理学检查;检测对气管黏膜树突状细胞(DC)数量(OX-62),成熟度:以CD80,趋化因子激素受体7(CCR7)阳性表达面积表示及抗原识别受体Toll样受体2(TLR2)和TLR4的影响;对鼻通道淋巴组织细胞CD4+,CD8+进行相应的检查。结果:与模型组比较,预防性中药对病毒载量及肺组织病理学有显著的抑制作用,流感病毒感染小鼠气管黏膜OX-62增加,冰香散(雾化)能使流感病毒感染鼠DC数降低,CD80和CCR7明显下降,而病毒唑和银翘散制剂能提升CCR7阳性表达面积,流感病毒感染鼠黏膜TLR2,TLR4表达增强,冰香散能抑制流感病毒感染鼠TLR2的表达,冰香散滴鼻给药能提高CD4+,CD8+的水平,均具有明显的统计学差异(P<0.05,P<0.01)。结论:三类防治流感的中药均有较好的抑制流感病毒作用,其作用可能与其调节呼吸道黏膜免疫作用有关。

Objective： To investigate the antiviral activity against influenza virus and mucosal immunoregulation mechanism of preventive herbs. Method： Fifty-six female SPF BALB / C mice were randomly divided into modeled group（ 48） and normal group（ 8）. Mice influenza virus（ H1N1 / FM /1） infection models were established. The mice after successfully modeling were randomly divided into model group,Virazole group（ oral administration,100 mg·kg- 1）,Yinqiao powder group（ oral administration,40 g·kg- 1）,Yupingfeng powder group（ oral administration,60 g·kg- 1）,Bingxiang powder atomization group（ 0. 6 g·kg- 1） and nasal drops group（ 0. 03 g·kg- 1）,ig administration,and sacrificed after 5 days. Viral load was determined,and lung tissue pathology examination was simultaneously done. Its influence on tracheal mucosal dendritic cells（ DC） count（ OX-62）,maturity CD80,chemokine receptor 7（ CCR7） positive expression area,Toll-like receptor 2（ TLR2）and TLR4（ pathogen recognition receptors） was detected. Corresponding tests of lymphoid tissue cells CD4＋and CD8＋in nasal cavity were conducted. Result： Compared with the model group,preventive herbs had significant inhibitory effect on viral load and pulmonary histopathology. Tracheal mucous OX-62 in mice infected with influenza virus was increased. Bingxiang powder atomization group could decrease DC count in mice infected with influenza virus,significantly decrease CD80 and CCR7. While virazole and Yinqiao powder Preparation could increase CCR7 positive expression area,increase mucous TLR2 and TLR4 expressions in mice infected with influenza virus. Bing xiang powder preparation was able to inhibit the expressions of TLR2 in mice infected with influenza virus. Bing xiang powder nasal drops could increase CD4＋,CD8＋levels,with statistically significant differences（ P〈0. 01,P〈0. 05）. Conclusion： Three preventive herbs for influenza virus have good effect against influenza virus,whose mechanism may be associated with regulating mucosal immunity in respiratory tract.