广西莪术多糖对2型糖尿病大鼠的降血糖作用

Hypoglycemic Effect of Curcuma kwangsiensis Polysaccharides on Type 2 Diabetes Mellitus Rats

目的:探讨广西莪术多糖(CKP)对2型糖尿病(T2DM)大鼠血糖的影响。方法:采用高糖高脂联合腹腔小剂量注射链脲佐菌素(STZ,45 mg·kg-1)的方法诱导T2DM模型,将造模成功的糖尿病大鼠随机分为4组:糖尿病模型组,CKP低、中、高剂量组(0.5,1.0,2.5 g·kg-1),另取10只正常大鼠设为正常组。给药组每天ig给药1次,连续给药6周;模型组和正常组则ig给予同体积的生理盐水。给药6周后,空腹检测大鼠血糖(FBG),胰岛素(INS),总胆固醇(TC),甘油三酯(TG)的水平;通过观察胰腺HE染色及胰腺免疫组化;检测胰岛β细胞Fas蛋白的表达。结果:与正常组大鼠相比,模型组大鼠血糖,TG,TC水平明显增高,且胰腺Fas蛋白表达增高,均具有统计学意义(P<0.05,P<0.01);与模型组比较,CKP给药组均可降低糖尿病大鼠的血糖,TC和TG水平;减少胰腺Fas蛋白表达,减少胰岛β细胞凋亡(P<0.05,P<0.01)。结论:广西莪术多糖降低2型糖尿病大鼠血糖,其作用可能是通过保护胰岛β细胞,减少其凋亡有关。

Objective： To investigate the hypoglycemic effect of Curcuma kwangsiensis polysaccharide（ CKP） on type 2 diabetes mellitus（ T2DM） rats. Method： T2 DM models were induced through high-sucrose and high-fat diet combined with low-dose abdominal injection of streptozotocin（ STZ,45 mg·kg- 1）. The successfully modeled rats were randomly divided into 4 groups： T2 DM model group,CKP low dosage group（ CKP-LG）,CKP middle dosage group（ CKP-MG） and CKP high dosage group（ CKP-HG）. Another 10 normal rats were selected as normal group. CKP groups with different dosages（ 0. 5,1. 0,2. 5 g·kg- 1·d- 1） were ig administered continuously for 6 weeks,once a day. The model group and normal group received ig normal saline of the same volume. After 6weeks of administration,fasting blood glucose（ FBG）,insulin（ INS）,total cholesterol（ TC）,and triglyceride（ TG） levels were detected in rats,and pathological change of pancreas was evaluated by hematoxylin and eosin（ HE） staining and immunohistochemistry,Fas protein expressions of pancreatic β cells were detected. Result：Compared with the normal rats,FBG,TG,and TC levels were increased significantly in rats of model group,and pancreas Fas protein expressions were increased,with statistically significant difference（ P〈0. 05,P〈0. 01）,Compared with the model group,CKP groups could reduce FBG,TC and TG levels of T2 DM rats,and reduce pancreas Fas protein expressions and reduce apoptosis of pancreatic β cells,with significant difference（ P〈0. 05,P〈0. 01）. Conclusion： The hypoglycemic effect of CKP on T2 DM rats may be achieved by protecting pancreatic βcells and reducing their apoptosis.