共表达双基因Adv CD40L-IRES2-ICOSL对小鼠乳腺癌的实验研究

Co-expression of pairs of genes Adv CD40L-IRES2-ICOSL experimental study of breast cancer in mice

目的探讨CD40L-IRES2-ICOSL腺病毒载体对小鼠乳腺癌模型的治疗作用。方法利用基因转染技术,分别构建腺病毒载体,建立小鼠乳腺癌动物模型,随机分为4组,2周后于瘤体内分别注射转染有空载体及不同的重组载体的乳腺癌细胞,观察组织病理改变、肿瘤体积、重量变化和荷瘤小鼠的生存时间。结果 CD40L组、ICOSL组、CD40L-IRES2-ICOSL组肿瘤内淋巴细胞浸润明显增多。与对照组相比,CD40L组、ICOSL组肿瘤生长减缓,体积减小,荷瘤生存期延长,两组间均有显著差异(P<0.05),而CD40L-IRES2-ICOSL组尤为显著(P<0.01);CD40L组与ICOSL组相比则无显著差异。结论共表达双基因Adv CD40L-IRES2-ICOSL治疗小鼠乳腺癌动物模型,能直接杀伤肿瘤,抑制肿瘤生长,延长荷瘤动物的生存期。

【Objective】To explore the role of CD40L-IRES2-ICOSL adenovirus vector treatment of breast cancer in mice. 【Methods】We used gene transfection techniques to construct the recombinant adenovirus vector of CD40 L, ICOSL,CD40L-IRES2-ICOSL. Select the 80 BALB/c mice, mice with MCF-7 human breast cancer cell lines to establish animal models of breast cancer were randomly divided into control group, CD40 L group, ICOSL group, CD40L-IRES2-ICOSLgroup（n= 20）. To the intratumoral injection transfected with empty vector and there combinant vector of breast cancer cells after two weeks to observe the pathological changes in tumor volume, weight change and the survival time of tumor-bearing mice.【Results】CD40L group, ICOSL group, CD40L-IRES2-ICOSL group within the tumor infiltration of lymphocytes increased significantly. Compared with the control group, CD40 L group, the ICOSL group tumor growth slowed, the volume decreases, prolonged survival of tumor-bearing, there were significant differences between the two groups（P〈0.05）,while CD40L-IRES2-ICOSL group is particularly significant（P〈0.01）; CD40 L group and ICOSL compared no significant difference.【Conclusion】Co-expression of two gene Adv CD40L-IRES2-ICOSL treatment of mice with breast cancer animal models, can directly kill the tumor, inhibiting tumor growth, prolong the survival time of tumor-bearing animals.