晶状体蛋白αb在结直肠癌组织中的表达及其临床意义

Expression of Alpha B-Crystallin in Colorectal Cancer and Its Clinical Significances

目的探讨晶状体蛋白αb(Cryab)在结直肠癌组织中的表达及其临床意义。方法收集兰州大学第一医院早复发和晚复发的结直肠癌标本10对,用RT-PCR法检测Cryab m RNA在早复发和晚复发患者中的表达差异;另收集兰州大学第一医院本部及东岗院区2005年1月至2008年12月期间100例行根治性手术切除患者的结直肠癌组织及其相应的癌旁组织,用免疫组织化学SP法检测100例结直肠癌组织和癌旁组织中Cryab蛋白的表达,并分析Cryab蛋白表达与结直肠癌患者的年龄、性别、肿瘤数目、肿瘤大小、TNM分期、分化程度、淋巴结转移及浸润深度的关系。使用Cox回归方法分析Cryab蛋白表达对结直肠癌患者预后的影响。结果 1 Cryab m RNA在早复发患者中的表达明显高于晚复发患者(P<0.05)。2 Cryab蛋白在结直肠癌组织中的表达明显高于其在癌旁组织中的表达(P<0.001)。3 Cryab蛋白表达与结直肠癌患者的肿瘤大小、分化程度、浸润深度、淋巴结转移及TNM分期有关(P<0.05),而与患者的性别、年龄及肿瘤数目无关(P>0.05)。4单因素分析结果显示,肿瘤大小、淋巴结转移及Cryab蛋白表达是结直肠癌患者总生存时间和无瘤生存时间的影响因素(P<0.05);进一步的Cox回归分析结果显示,淋巴结转移及Cryab蛋白表达是影响结直肠癌患者预后的独立危险因素。5 Cryab蛋白高表达患者的总生存率及无瘤生存率均明显低于Cryab蛋白低表达患者,差异有统计学意义(P=0.003,P=0.005)。结论 Cryab蛋白的高表达与结直肠癌的侵袭和发展有关,其可能参与了结直肠癌的演进,Cryab可能作为判断结直肠癌恶性程度和预后的指标。

Objective To explore the expression of Cryab in human colorectal cancer tissue and its clinical significances.Methods Ten pairs of colorectal cancer specimens of early recurrence and late recurrence in the First Hospital of Lanzhou University were collected,the Cryab mRNA expression was detected by RT-PCR.The colorectal cancer tissues and corresponding adjacent tissues of 100 patients underwent radical resection from January 2005 to December 2008 in the Donggang Branch of The First Hospital and The First Hospital of Lanzhou University were collected,the expression of Cryab protein in the colorectal cancer tissues and corresponding adjacent tissues of 100 patients with colorectal cancer were detected by immunohistochemistry SP technique.The relation between the expression of Cryab protein and the age,gender,number of tumors,tumor size,TNM stage,degree of differentiation,lymph node metastasis,or depth of invasion of the patients was analyzed.The influence of Cryab expression on prognosis of patients with colorectal cancer was evaluated by univariate and multivariate analysis.Results ① The expression of Cryab mRNA in the colorectal cancer tissues with early recurrence was significantly higher than that in the colorectal cancer tissues with late recurrence（P0.05）.② The expression of Cryab protein in the colorectal cancer tissues was significantly higher than that in the corresponding adjacent tissues（P0.05）.③ The over-expression of Cryab protein in the colorectal cancer tissues was correlated with the tumor size,degree of differentiation,lymph node metastasis,or TNM stages（P0.05）,but not correlated with the age,sex,and number of tumors（P〈0.05）.④ The lymph node metastasis and expression of Cryab protein were the independent risk factors for the prognosis of patients with colorectal cancer.⑤ The overall survival and disease free survival of patients with high-expression of Cryab protein were significantly lower than those in the patients with lower-expression of Cryab protein.Conclusions The over-expression of Cryab is closely related to the invasion and progression of colorectal cancer,they might be together involved in the progression of colorectal cancer.Cryab might be a good marker for monitoring the malignant degree and the prognosis of colorectal cancer.