铜绿假单胞菌耐消毒剂基因检测

Detection of disinfectant-resistant gene in Pseudomonas aeruginosa isolates

目的了解深圳市人民医院临床分离的铜绿假单胞菌耐季胺盐类消毒剂基因(qac)的检出率及基因型。方法收集深圳市人民医院近几年铜绿假单胞菌临床分离菌株63株,应用PCR法检测菌株的qacA/B、qacC、qacG、qacJ和qacE△1基因。结果63株铜绿假单胞菌中,qacE△1基因阳性51株(80.9%),qacA/B基因阳性5株(7.9%),其余的基因型未检出。结论我院临床分离的铜绿假单胞菌中耐季铵盐类消毒剂基因检出率较高,主要为qacE△1型。

Objective To understand the occurrence and genotype of quaternary ammonium compound（qac）resistance gene in Pseudomonas aeruginosaisolates from Shenzhen People＇s Hospital.Methods 63 strains of clinical Pseudomonas aeruginosaisolates were collected.PCR was used for the detection of qacA/B,qacC,qacG,qacJ and qacE△1genes.Results Of all these strains,80.9%（51/63）was found carring qacE△1gene,five strains（7.9%）were found harbouring qacA/B gene.None of the strains were found carring qacC,qacG or qacJ gene.Conclusion The detection rate of qac gene in Pseudomonas aeruginosa from our hospital is high and the dominant type is qacE△1.