潘氏细胞在昆明小鼠弓形虫病发病过程中的变化

The Change of Paneth Cells in the Toxoplasmosis of Kunming Mice

每只昆明小鼠灌胃1×104个弓形虫卵囊,分别在灌胃后1,3,5,6 DAI(days after inoculation),采用H&E方法和免疫组织化学方法对小鼠回肠潘氏细胞的变化以及弓形虫在回肠的分布和数量进行研究,探讨潘氏细胞(paneth cells,PCs)在小鼠弓形虫病感染期间的变化。结果发现,回肠隐窝总数、含PCs的隐窝数、PCs总数及颗粒总数呈先增加后迅速减少至消失的趋势,回肠后段表现明显,6 DAI各参数与对照组比较差异显著(P<0.05)。弓形虫在回肠中的分布面积呈增大趋势,6 DAI与1 DAI、3 DAI相比差异显著(P<0.05)。该研究结果表明,弓形虫卵囊感染昆明小鼠可减少回肠潘氏细胞及其分泌颗粒的数量,为以小肠潘氏细胞为基础的弓形虫病的防治提供了实验依据。

1× 104 Toxoplasma gondii oocysts were fed to Kunming mice orally. Clinical symptoms and lesions were observed on 1, 3, 5, 6 days after inoculation （DAI）. Ileal lesions, the distribution and quantity of T. gondii, the change of paneth cells （PCs） and granules were checked by H＆E and immunohistochemical staining. The results showed that the total number of crypts, crypts containing PCs, PCs and granules in ileum were increased first, then decreased explosively, especially in the distal ileum, compared to the control group （P〈0.05）. The positive area of T. gondii in the ileum was increased. Compared with 1 DAI or 3 DAI, the positive area of T. gondii was the highest at 6 DAI （P〈0.05）. The results indicated that T. gondii oocysts had a significant influence on PCs of Kunming mice, which providing an experimental basis for the prevention and treatment oftoxoplasmosis based on small intestine PCs.