毛菊苣根-种子不同配伍的乙醇提取物对小鼠肝损伤的保护作用

Protective effects of ethanol extracts in different proportion of roots and seeds from Cichorium glandulosum Boiss.et Huet against acute liver injury in mice

目的探讨毛菊苣(MJJ)根和种子2种药用部位不同比例的配伍对小鼠化碳性肝损伤和免疫性肝损伤的保护作用。方法将MJJ根(root,R)和种子(seed,S)按不同(R∶S,0∶1,1∶1,1∶2,1∶3,2∶1和1∶0)质量比例进行配伍,再以8倍体积分数70%乙醇提取后获得6种配伍提取物。建立四氯化碳(CCl4)致小鼠化学性肝损伤模型和卡介苗(BCG)加脂多糖(LPS)致小鼠免疫性肝损伤模型,将小鼠随机分为空白对照组、模型组、联苯双酯组和MJJ不同比例的配伍提取物组,各组以20mL·kg-1灌胃给药。检测各组小鼠血清中天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活性,计算脏器系数,观察肝组织病理性变化。结果 MJJ根-种子以质量比1∶0(R1S0)、1∶1(R1S1)和2∶1(R2S1)配伍的乙醇提取物能明显降低CCl4致小鼠急性化学性肝损伤模型中血清AST和/或ALT活性;MJJ根-种子以R1S1和R2S1配伍的乙醇提取物亦能明显降低BCG+LPS致急性免疫性肝损伤模型中血清AST和ALT活性;MJJ根-种子以R1S1和R2S1配伍的乙醇提取物还能改善模型小鼠肝细胞坏死、变性、炎性细胞浸润等病理现象。结论 MJJ根和种子不同配伍的提取物对肝损伤有不同的改善作用,根-种子以R1S1和R2S1配伍组可有效保护肝功能和缓解肝病理变化。

Objective To study the protective effects of Cichorium glandulosum Boiss. et Huet （MJJ） different proportion of roots （R） and seeds（S） against acute hepatic injury in mice. Methods Kunming mice were randomly divided and pretreated with MJJ extracts by oralgavage 20 mL· kg 1. There were totally6 proportions of roots and seeds as0 ： 1,1 ： 1,1 ： 2,1 ： 3,2 ：1 and 1 ： 0 （R - S） according to the amounts. Carbon tetrachloride （CC14）-induced-acute hepatic damage and Bacillus Calmette-Guerin （BCG） and lipopolysaccharide （LPS） induced-immunological liver injury were both established in mice. Levels of aspartate aminotrans- ferase （AST） and alanine aminotransferase （ALT） in blood were detected to evaluate hepatic injury. Liver histopathological ob- servation was perfonmed for the hepatic injury evaluation degree. Results R1 S1 and R2 S1 extract significantly decreased the levels of ALT and AST in serum of the 2 liver injury models compared with model groups. Moreover, histopathological results also showed the obvious protection of liver injury in R1S1 and R2S1 extract groups. Conclusion Different proportions of MJJ root-seed extract may influence the liver damage in mice with different degrees and especially, R1S1 and R2S1 extracts showed protective effects on hepatotoxicity.