氧化鱼油对草鱼幼鱼抗氧化酶mRNA相对表达量的影响

Effects of Oxidized fish Oil on Relative Expression of Antioxidant Enzymes mRNA in Juvenile Grass carp(Ctenopharyngodon idellus)

旨在研究氧化鱼油对草鱼幼鱼抗氧化酶mRNA相对表达量的影响。以过氧化值(POV)为9.07(新鲜鱼油)、49.72、144.64、345.45 meq O_2/kg的鱼油配制4种等氮等能的草鱼配合饲料,分别为对照组、轻度氧化组、中度氧化组和重度氧化组。挑选草鱼幼鱼(30±2.68)g 204尾,随机分成4组,每组3重复,分别饲喂上述4种草鱼饲料,饲养8周:以β-actin为内参基因,采用RT-PCR法检测组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)和谷胱甘肽合成连接酶催化亚基(GCLC)mRNA相对表达量。结果表明:氧化鱼油显著降低3种组织SOD mRNA表达量(9<0.05);显著降低肾脏和背肌CAT mRNA表达量(P<0.05);轻度和中度氧化鱼油组GPx mRNA表达量显著升高(P<0.05);氧化鱼油显著降低3种组织GCLC mRNA表达量(P<0.05)。氧化鱼油对草鱼幼鱼抗氧化酶mRNA相对表达量的影响具有组织差异性,鱼油氧化程度越严重,对SOD、CAT和GCLC mRNA表达的抑制作用越强。

This experiment was conducted to study the influence of different degrees of oxidized fish oil on relative expression of antioxidant enzymes mRNA in juvenile grass carp （Ctertopharyngodon idellus）. Using peroxide value （POV） was 9.07 （fresh fish oil）, 49.72, 144.64, 345.35 meqO2/Kg fish oil to formulate different oxidation degree feed, including control group, mild oxidation group, moderate oxidation group and severe oxidation group. A total of 204 fish of initial body weight （30±2.68） g were randomly divided into 4 groups, each group was triplicate. This experiment was lasted for 8 weeks. Using β-actin gene as internal reference and RT-PCR assay to detect the influence of mRNA relative expression of superoxide dismutase （SOD）, catalase （CAT）, glutathione peroxidase （GPx） and glutathione synthesis ligase catalytic subunit （GCLC） in hepatopanereas, kidney and dorsal muscle of tested fish. Oxidized fish oil significantly decreased the expression of SOD mRNA in three tissues （P〈0.05）; three degree of oxidized fish oil significantly reduced CAT expression mRNA of kidney and muscle; and the GPx mRNA expression were significantly increased in mild and moderate oxidation of fish oil groups; oxidized fish oil significantly reduced the expression of GCLC mRNA in hepatopancreas, kidney and dorsal muscle （P〈0.05）. In this study, the different responses of relative expression of antioxidant enzyme mRNA were found in hepatopancreas, kidney and dorsal muscle that the fish was fed with by three degree of oxidized fish oil. With the increasing degree of oxidized fish oil, the inhibition of relative expression of SOD, CAT and GCLC mRNA was greater.