猫杯状病毒的分离鉴定及超变区基因序列分析

Isolation,identification and hypervariable region analysis of a feline calicivirus isolated from a cat

为了解猫杯状病毒(FCV)的生物学特性,本研究对猫体内分离到的一株FCV HRB-SS株进行了病毒学、分子生物学鉴定和遗传学分析。结果显示该分离株能够在猫肾细胞系(CRFK)中产生明显的细胞病变,形成蚀斑。FCV的VP1基因超变区核苷酸序列分析结果显示,HRB-SS分离株与疫苗株CVXPOLYCAP同源性最高,为78.2%,而与中国分离株CH-JL和GD的同源性最低,仅为67.9%和68.8%;推导氨基酸序列分析结果显示,HRB-SS分离株与12Q087-5、UTCVM-NH12和FCLF4的同源性最高,为86.7%,而与中国分离株的同源性仅为73.9%。系统发育树分析结果显示,HRB-SS分离株与中国分离株亲缘关系较远。本研究结果表明,FCV HRB-SS与我国FCV具有不同的遗传起源。

To investigate the biological characteristics of feline calicivirus （FCV） HRB-SS isolated from a diseased cat, the isolate was identified by the methods of virology, molecular biology and bioinformatic. The results showed that the virus was able to induce cytopathic effect and the plaque when it infected the CRFK cells. Nucleotide sequence analysis results of the hypervariable region showed that the FCV HRB-SS was closely related to the vaccine strains CVXPOLYCAP （78.2%）, but with the lowest homology to the China isolates of CH-JL （67.9%） and GD （68.8%）. The analysis results of deduced amino acid sequence indicated that the FCV HRB-SS had the highest homology （86.7%） with 12Q087-5, UTCVM-NH12 and FCLF4, and had only 73.9% homology with China isolates. Phylogenetic tree analysis manifested that the FCV HRB-SS and China isolates were located in different cluster. The present study suggested that the FCV HRB-SS and the China isolates originated from different ancestors.