高效液相色谱-紫外检测器法测定高脂饮食小鼠血清中槲皮素含量

Determination of Quercetin in Serum of High Fat Diet-Fed Mice by HPLC-UV

目的：建立用高效液相色谱-紫外检测器检测法测定饮食诱导的肥胖小鼠血清中槲皮素含量的方法。方法：色谱柱：Eclipse XDB-C18柱（4.6 mm×250 mm,5μm）;流动相：乙腈-0.2%甲酸溶液（40∶60,V/V）;检测波长：360 nm;柱温：30℃;流速：0.50 m L/min。结果：槲皮素在2.5～40μg/m L质量浓度范围内呈良好的线性关系（r=0.999 1）,平均回收率为96.0%,相对标准偏差为10.98%（n=5）。结论：该方法具有良好的回收率和重复性。可以对血清中的槲皮素实现简便、快速、准确的检测。

The quercetin content of serum from diet-induced obese mice was determined by high-performance liquid chromatography fitted with an ultraviolet detector（HPLC-UV） and an Agilent Eclipse XDB-C18 column（4.6 mm × 250 mm, 5 μm）. The mobile phase was a mixture of acetonitrile-0.2% formic acid（40：60, V/V） with a flow rate of 0.5 m L/min, and the detection wavelength was 360 nm. There was a good linear relationship for quercetin in the range of 2.5–40 μg/m L（r = 0.999 1）. The average recovery rate for quercetin was 96.0% with relative standard deviation（RSD） of 10.98%（n = 5）. This method could be used to determine quercetin in serum with good recovery rate and repeatability. It was a simple, rapid and accurate analytical method.