反相液相色谱中lgI对极性小分子溶质色谱行为的表征

Characterization of Chromatographic Behavior of Polar Solutes of Small Molecules with lgI in Reversed-Phase Liquid Chromatography

以极性溶质正链醇同系物及芳香醇同系物的实验数据对反相液相色谱中计量置换表征参数lgI进行了多方面验证 ,并将lgI值与溶质的其他物理化学参数做了比较 ,证明了lgI在限定条件下分别可作为溶质的种类和大小、流动相中置换剂、所选用固定相特性及有关溶质分离选择性大小的表征参数 ,也证明了极性溶质体系的lgI亦具有热力学平衡常数的性质。

A new characterization parameter lg I , affinity constant, in reversed phase liquid chromatography (RPLC) has been tested at several aspects with experimental data of polar n alkyl alcohol homologoues and aromatic alcohol homologoues. The lg I values were compared with other physicochemical parameters of corresponding solutes, such as hydrophobic fragmental constant (lg f ), van der Waals volume ( V W ), molecular connectivity ( χ ). It was proved that lg I was also used for the characterization of solutes, mobile and stationary phases in RPLC. Moreover, it was also confirmed that lg I has the property of thermodynamic equilibriam constant.