摘要
目的:建立酸枣仁胶囊中菝契皂苷元的HPLC-ELSD含量测定方法。方法:采用反向高效液相色谱-蒸发光散射检测法。色谱柱为Waters Symmetry C18色谱柱(4.6mm×150mm,5μm),流动相为甲醇-水(90∶10),流速:1.0m L/min;柱温30℃;检测器漂移管温度85℃,载气流量1.8L/min。结果:菝契皂苷元在0.326-6.520μg范围内线性关系良好(相关系数为0.9996)。平均加样回收率为99.92%,RSD为2.06%(n=6)。结论:该方法操作简便,专属性强,重现性好,可用于酸枣仁胶囊中菝契皂苷元的含量测定。
Objective:To develop a HPLC-ELSD method to determine Sarsasapogenin in Wild Jujube Seed Capsules. Methods The HPLC method was performed on an Waters Symmetry C18column(4.6mm×150mm,5μm) with the mobile phase consisted of methanolwater(90∶10),at the flow rate of 1.0m L·min-1;The temperature of the drift tube was 85℃ and the nebulizer nitrogen flow rate was 1.8L·min-1. Results The liner range of Sarsasapogenin was 0.326-6.520μg(r=0.9996). The average recovery was 99.92%,RSD 2.25%(n=6).Conclusion This method is simple,rapid,specialty,reproducible and suitable to test Sarsasapogenin in Wild Jujube Seed Capsules.
出处
《北方药学》
2015年第11期5-6,共2页
Journal of North Pharmacy