高效液相色谱法测定胃康片中呋喃唑酮、甘珀酸钠和盐酸小檗碱

Determination of Furazolidone, Carbenoxolone Sodium and Berberine Hydrochloride in Wei Kang Tablets by Reversed-Phase High Performance Liquid Chromatography(RP HPLC)

用高效液相色谱 (HPLC)梯度洗脱法同时测定复方胃康片中呋喃唑酮、甘珀酸钠和盐酸小檗含量。使用No va PakC18柱 ,以乙腈和 0 0 2mol/L磷酸盐缓冲溶液 (pH 7 0 )为流动相 ,3,5 二硝基苯甲酸作为内标溶液 ,使用UV检测器 ,在 2 5 4nm处对样品中上述 3种成分进行测定。测定结果表明 ,呋喃唑酮在 14 1 2mg/L～ 12 70 8mg/L、甘珀酸钠在 10 0 6mg/L～ 90 5 4mg/L、盐酸小檗碱在 99 2mg/L～ 892 8mg/L时其相对峰面积 (标准品与内标的峰面积之比 )与进样质量浓度有良好的线性关系 ,其线性相关系数分别为r =0 9997,r =0 9995和r =0 9991。测定结果显示 :该方法灵敏可靠 ,专属性强 ,且具有快速、简便、重现性好的特点。

An HPLC method with gradient elution was developed to separate and determine furazolidone, carbenoxolone sodium and berberine hydrochloride in Wei Kang Tablets on Nova Pak C 18 column(150 mm×3 9 mm i d , 4 μm), using acetonitrile phosphate buffer solution (pH 7 0)as mobile phase and 3,5 dinitrobenzoic acid as the internal standard. Detection was performed with UV detector at 254 nm. The calibration curves were linear within the ranges of 141 2 mg/L - 1?270 8 mg/L for furazolidone( r = 0 999?7 ), 100 6 mg/L -905 4 mg/L for carbenoxolone sodium( r = 0 999?5 ) and 99 2 mg/L -892 8 mg/L for berberine hydrochloride( r = 0 999?1 ) The recoveries and RSDs were 99 5% and 1 38% for furazolidone, 100 3% and 1 73% for carbenoxolone sodium, 97 3% and 1 97% for berberine hydrochloride respectively. The results show that this method is simple, rapid, specific, accurate and reproducible.