气相色谱法测定葡萄中烯唑醇农药的残留量

Determination of Diniconazole Residue in Grape by Gas Chromatography

用气相色谱法测定葡萄中的烯唑醇农药残留量。样品中的烯唑醇经丙酮提取 ,液 液分配和固相萃取小柱净化 ,用毛细管色谱柱分离 ,气相色谱 电子捕获检测器 (GC ECD)测定 ,外标法定量。方法的检测限为 0 0 1mg/kg ,回收率 >85 % ,相对标准偏差 <5 %。

An efficient method was developed for the determination of diniconazole in grape by gas chromatography. The diniconazole residue in the sample was extracted by acetone. The extract was cleaned up by liquid liquid extraction and then solid phase extraction. The diniconazole residue was separated completely from other impurities in the sample through SPB TM 1701 capillary column (30 m×0 53 mm i d ×1 0 μm) and determined by an electron capture detector. The recoveries ranged from 85 8% to 94 7% when the added levels were from 0 01 mg/kg to 1 00 mg/kg. The RSD was within 5% and the limit of detection was 0 01 mg/kg.