HIV感染者淋巴细胞相关趋化因子的分泌与分布

Secretion and distribution of chemokine associated with lymphocytes in peripheral superficial lymph nodes of HIV infected individuals

目的 研究不同感染阶段HIV感染者外周浅表淋巴结内CCL19、CCL21、CXCL12和CXCL13的分泌与分布,探讨HIV感染对淋巴细胞在淋巴结内定位与生存的影响.方法 选取不同HIV感染阶段的患者43例,分为HIV感染无症状组和AIDS组,采用免疫组织化学方法检测淋巴结中CCL19、CCL21、CXCL12和CXCL13的分泌及分布情况,非HIV感染者12例为对照.结果 HIV感染无症状者组与对照组相比,外周浅表淋巴结内仅CCL21表达偏低,差异有统计学意义,而CCL19、CXCL12及CXCLI3差异均无统计学意义;AIDS组与对照组相比,外周浅表淋巴结中CCL19、CXCL13表达升高,差异有统计学意义,而CCL21、CXCL12差异均无统计学意义,与无症状组相比,相关趋化因子表达均升高,差异有统计学意义;HIV感染至AIDS期后,外周浅表淋巴结中各个趋化因子的分布没有明显的区域界线.结论 HIV感染者外周浅表淋巴结中相关趋化因子的改变可能是机体代偿和淋巴结结构破坏的结果,而这种变化又进一步促进了免疫功能的破坏和CD4＋T细胞的减少.

Objective To evaluate the effect of HIV infection upon distribution and survival of lymphocytes in peripheral superficial lymph nodes through checking CCL19,CCL21,CXCL12 and CXCL13.Methods 43 HIV-infected individuals were recruited into this investigation,and were divided into two groups,asymptomatic HIV carriers or AIDS patients,basing on CD4＋ T cell count by flow cytometry and clinical symptom.Then,immunohistochemistry was used to detect secretion and distribution of chemokines,data was analyzed with statistical software.Results Comparing to control group,only CCL21 expression decreased in peripheral superficial lymph nodes of asymptomatic chronic HIV-infected individuals,CCL19 and CXCL13 expression markedly increased in AIDS patients.And secretion level of CCL19,CCL21,CXCL12 and CXCL13 in peripheral superficial lymph nodes of asymptomatic carriers is profoundly less than AIDS patients.These differences were significant.Moreover,domain boundary of these chemokines was indefinite in AIDS patients.Conclusions Alteration of chemokines in peripheral superficial lymph nodes of HIV-infected individuals,which promote failure of immune function and depletion of CD4 ＋ T cells,could result from compensatory and adaptive response of body and disorganization of lymph nodes.