黑龙江省大豆菌核病菌核盘菌遗传多样性分析

Genetic diversity of Sclerotinia sclerotiorum from Heilongjiang Province

了解黑龙江省不同地区侵染大豆核盘菌菌株分离物间的主要特性差异,利用PDA培养基对核盘菌进行分离和纯化,同时利用RAPD和r DNA-ITS标记方法对核盘菌进行遗传多样性分析,获得了50株纯化的核盘菌,用RAPD标记确定的遗传相似系数范围为0.54–0.98,平均相似系数为0.76,说明供试的核盘菌菌株的基因型具有一定的差异。对50个测定序列有差异的32个核盘菌ITS和5.8S r DNA片段的多序列对位分析,在ITS1区域的1–40bp种间变化较大,主要以碱基颠换和转换为进化形式。ITS2区域非常保守没有变异位点。黑龙江省核盘菌菌株在DNA水平上和ITS间隔区上具有较显著的遗传变异,显示出丰富的遗传多样性。

The main property difference of soybean Sclerotinia sclerotiorum isolates from Heilongjiang Province is studied Sclerotinia sclerotiorum was isolated and purified on PDA medium, and the RAPD and rDNA ITS methods were used to analyze the isolates. A total of 50 isolates of Sclerotinia sclerotiorum were obtained from diseased soybean. Using RAPD marker the genetic similarity coefficient ranged from 0.54 to 0.98, and the average value was 0.76, indicating that there were genetic difference among the isolates. Analyzing the sequence of the 5.85 region and nrDNA ITS of 32 Sclerotinia sclerotiorum isolates showed that there was large variation at the ITS1 region of 1-40bp, mainly in the form of base transversion and transition. There was no mutation at the ITS2 region of most of the isolates. There was significant genetic variation among Sclerotinia sclerotiorum isolates in Heilongjiang Province at the DNA level and iTS spacer region.