铁线莲上隐匿柄锈菌性孢子器和锈孢子器发育的组织学和细胞学观察

Histological and cytological observations of pycnial and aecial formation of Puccinia recondita on Clematis intricata

通过光学显微镜技术和电镜技术对铁线莲上隐匿柄锈菌Puccinia recondita性孢子器和锈孢子器发育进行了组织学和超微结构的观察。观察表明,铁线莲上隐匿柄锈菌性孢子器呈瓶状,内生有大量单核的性孢子梗和侧丝。性孢子梗顶端产生大量的性孢子,每形成一个性孢子后,都在性孢子梗上留下一环痕。性孢子长椭圆形,表面无特殊纹饰。铁线莲上隐匿柄锈菌锈孢子器呈杯状,其内生长大量平行排列的长形双核锈孢子梗,每个锈孢子梗由上向下连续产生多个锈孢子。锈孢子梗通过产生隔膜先形成锈孢子原体,再分隔形成锈孢子和间生细胞。随着锈孢子的进一步发育,间生细胞解体,锈孢子彼此分开。成熟的锈孢子表面产生有密集的瘤状结构。

Pycnial and aecial formation of Puccinia recondita on Clematis intricata are observed by means of light and electron microscopies. The pycnium of P. recondita is flask-shaped, producing a periphyses inside. Numerous pycniospores are generated successively mature pycniospore released from a pycniosporophores, an annular scar large number from the top was observed of monokaryotic pycniosporophores and of pycniosporophores in chain. After a on the top surface of a pycniosporophoreA pycniospore is ova producing numerous n shape with long, and d no special ornaments on the surface. An aecium of P. recondita is cylinder-like in shape, karyotic aeciosporophores that generate successively a number of aeciospores. An aeciosporophore divided to form an aeciospore initial, and a septum separated the initial into an immature aeciospores and an ntercalary cell, with the further development of aeciospores, the intercalary cell disintegrated and the spores separated from each other. The surface of a mature aeciospore is covered with numerous verrucas.