条形柄锈菌33号生理小种的分子标记

The molecular markers of Puccinia striiformis f. sp. tritici race CYR33

采用RAPD-SCAR分子标记技术,从300条RAPD随机引物中筛选到了对条形柄锈菌Puccinia striiformis f.sp.tritici33号生理小种特异的2条引物,将特异性片段回收、克隆和测序后(Gen Bank注册号为AB914691和AB914692),依据其序列设计出了2对引物S261F33/S261R33和S300F33/S300R33,能够特异性地从33号生理小种基因组DNA及发病小麦叶片总DNA中分别扩增出247bp和763bp的片段,其结果与采用常规的鉴别寄主法鉴定的结果一致。因此,这2对引物都可用于条形柄锈菌33号生理小种的快速鉴定与监测。

Two primers among 300 RAPD primers were obtained to specialize CYR33 of Puccinia striij：ormis f. sp. tritici using SCAR-PCR technique. Two specific DNA fragments of CYR33 were recovered from the agarose gel, cloned, and sequenced （GenBank Deposit Number： AB914691 and AB914692）. Based on the sequences, two specific primers （S261F33/S261R33, and S300F33/S300R33） were designed and they could amplify 247bp and 763bp specific bands from genomic DNA of CYR33 and tota DNA of wheat leaves infected by CYR33, respectively. The identification results using the two primer pairs were the same as those using differential host. Thus, the two primer pairs could be used for CYR33 identification and monitoring rapidly .