封闭抗体及纤溶酶原激活抑制物-1在自然流产中的临床应用

Clinical application of blocking antibody and plasminogen activator inhibitor-1 in spontaneous abortion

目的探讨血清中封闭抗体(BA)及纤溶酶原激活抑制物-1(PAI-1)水平在自然流产患者中的临床应用价值。方法采用酶联免疫吸附试验、免疫比浊方法对89例自然流产患者以及92例人工流产患者的血清,分别进行BA及PAI-1水平检测。结果与人工流产组比较,自然流产患者BA阳性率显著降低,而PAI-1水平显著增高,差异具有统计学意义(P<0.01,P<0.05);并且自然流产次数≤2次患者,BA阳性率为8%,而自然流产次数≥3次患者BA阳性率显著降低,仅为2%,差异具有统计学意义(P<0.05);PAI-1水平在自然流产次数≤2次患者为(76.43±7.45)ng/ml,而在自然流产次数≥3次患者显著增高为(101.19±13.35)ng/ml,差异具有统计学意义(P<0.01)。结论 BA及PAI-1对自然流产的早期预防、早期诊断及早期积极治疗有着重要的临床应用价值。

Objective To investigate the clinical application value of plasma blocking antibodies（ BA） and plasminogen activator inhibitor-1（ PAI-1） in the patients with spontaneous abortion. Methods The serum of 89 patients with spontaneous abortion and 92 patients with induced abortion were collected. BA and PAI-1 were detected using ELISA assay and turbidimetric inhibition immuno assay respectively. Results Compared with the induced abortion,the positive rate of BA of spontaneous abortion was significantly decreased,while the level of PAI-1 significantly increased and the differences had statistical significance（ P〈0. 01,P〈0. 05）; moreover,when the frequency of spontaneous abortion was ≤2,the the positive rate of BA was8%,and when the frequency of the spontaneous abortion ≥3,the positive rate of BA was significantly decreased,only 2%,the differences has statistical significance（ P〈0. 05）; the level of PAI-1 was（ 76. 43 ± 7. 45） ng / ml when the spontaneous abortion times was ≤2; the level of PAI-1 significantly increased to（ 101. 19 ± 13. 35） ng / ml when the spontaneous abortion times was ≥3 and the differences had statistical significance（ P〈0. 01）. Conclusion BA and PAI-1 have important clinical application value to early prevention,early diagnosis,early treatment in spontaneous abortion.