不同钛涂层表面结构对于成骨细胞增殖及分化的影响

Effects of different structures on titanium surface on osteoblast proliferation and differentiation

目的比较具有不同表面结构的钛涂层对于成骨细胞增殖及分化的影响,为钛表面处理探究更经济实用的方法。方法将直径10mm,厚度1mm的纯钛片按表面处理方法分为四组:STi:纯钛抛光组;MTi:喷砂酸蚀组;NTi-5:喷砂酸蚀后,后续碱热处理5小时组;NTi-10:喷砂酸蚀后,后续碱热处理10小时组。对各组表面显微结构、元素组成及润湿角进行观察分析;进一步检测不同组涂层对于前成骨细胞(MC3T3-E1)增殖与ALP活性的影响。结果MTi组表面为微米级微孔,直径3-5μm;NTi-5及NTi-10中,通过碱热处理,在微米级孔内引入了纳米级微孔,平均直径分别约为100nm(NTi-5)、200nm(NTi-10);在NTi-5及NTi-10中,具有生物活性的钙元素和钠元素被引入;表面润湿角测定结果表明,NTi-5具有最优的表面润湿性;体外细胞增殖实验表明:与MTi相比,同时具有微纳复合结构的NTi-5及NTi-10具有更好的促进成骨细胞增殖效果;ALP活性实验表明:NTi-5及NTi-10并没有因为引入纳米结构,降低对于促进成骨细胞分化的作用。结论同时具有微纳复合结构的钛涂层,具有更加良好的生物学活性,并且纳米孔直径在100nm左右最佳。

Objective To compare the effects of different structures on titanium surface on osteoblast proliferation and differentiation and to find an economic way of titanium surface modification. Methods Pure titanium plates（10mm X10 mm X1mm） were divided into four groups： STi： prepared by polishing step by step with sandpapers; MTi：prepared by bead blasted and acid etching on STi; NTi-5：prepared by alkali etching treatment on MTi for 5 hours; NTi-10：prepared by alkali etching treatment on MTi for 10 hours. Analyze the surface microstructures, chemical compositions and contact angles of each group and investigate the effects of different structure on osteoblast proliferation and differentiation. Results Micropits with diameters of 3-5μm appear on MTi, while hierarchical structure with micro/nanopits appear on NTi-5 and NTi-10. Besides, the bioactive elements（calcium and sodium） were induced onto the NTi-5 and NTi-10 surface. Among the contact angle results, NTi-5 shows the best result with average 27°. In the proliferation and differentiation assay, NTi-5 and NTi-10 are significantly better than other groups. Conclusion Hierarchical structure encompassing micropits and nanopits shows more biocompatibility and NTi-5 with nanostructures of 100 nm is the best.