核糖核酸的直接化学顺序分析

DIRECT CHEMICAL METHOD OF SEQUENCING GEL

本文根据Peattie(1979)方法系统而简明地介绍了RNA一级结构测定,包括原理、标记方法、化学修饰操作和顺序胶等。此外,还讨论了读胶常遇到的一些问题。作为一个例子,病毒RNA3′末端顺序结果的放射自显影图也作了简要说明。

The article is to introduce the method tor sequencing RNA on the basis of Peattie (1979) . It deals with the principles, labling means, the procedure of chemical modification and sequencing gel. In addition, reading gel as well as the 3'-end of viral RNA, as an example is discussed.