DRD1基因敲除小鼠繁殖模式分析及鉴定

Study on Breeding and Reproductive Modes for DRD1 Gene Knockout Mice

为获得纯合多巴胺D1受体(DRD1)基因敲除小鼠,试验引进敲除杂合子小鼠进行饲养繁殖,依据孟德尔遗传定律,子代应包含三种基因型(敲除纯合子、敲除杂合子及野生型)小鼠,需进行PCR鉴定。结果显示,DRD1基因敲除小鼠的饲养繁殖获得成功,目前已获得大量基因敲除纯合子小鼠。通过对DRD1基因敲除小鼠不同繁殖模式下小鼠的分娩数、总产仔数、平均每胎产仔数和成活数比较,发现杂合子的繁育能力最强,纯合子之间进行交配繁育能力最低。试验表明,正确的饲养繁殖以及可靠的鉴定方法可得到DRD1基因敲除鼠,且DRD1敲除杂合子小鼠较敲除纯合子小鼠繁育能力强,提示DRD1基因可能影响动物繁育能力,研究结果为动物繁育研究提供参考思路。

To breed and identify DRD1 gene knockout mice, Heterozygote mice were bred and reproduced. According to Mendelian laws,wild type, heterozygote genotype, and homozygote genotype would appear in the offspring of parents. Therefore, genome DNA extracted from the mice＇s tails were subjected to PCR test for genotype identification. The findings showed that the breeding and reproducing were successful and homozygous genotype mice were acquired. The deliveries, litter size, birth per labor number, averaged litter survival number were compared between different mating types with DRD1 gene knockout mice. It＇s found heterozygote mating type showed higher reproduction ability. And homozygous mating type showed lower reproduction ability. Appropriate methods of feeding, breeding and identifying are important for obtaining gene knockout mice and protecting species. And heterozygous DRD1 gene knockout mice showed higher reproduction ability than homozygou, which meant DRD1 gene might have an effect on reproduction ability. The results will provide new ideas for animal fetility.