铁蛋白对MPP^+诱导MES23.5细胞损伤的作用

EFFECT OF FERRITIN ON MES23.5 CELL DAMAGE INDUCED BY 1-METHYL-4-PHENYLPYRIDINIUM

目的探讨外源性铁蛋白(Ferritin)对1-甲基-4-苯基吡啶阳离子(MPP+)诱导的MES23.5细胞损伤的作用。方法用四甲基偶氮唑盐(MTT)比色法筛选MPP+最适造模浓度;分别应用MPP+、Ferritin、Ferritin+MPP+处理MES23.5多巴胺能神经细胞,然后应用CCK8试剂盒检测细胞存活率,流式细胞术检测细胞线粒体跨膜电位(△Ψm)。结果 MPP+处理MES23.5细胞后,细胞存活率和△Ψm均下降,与对照组相比差异具有统计学意义(F=143.044、70.924,P<0.01)。Ferritin预处理4h可明显抑制MPP+导致的细胞存活率和△Ψm的下降(F=51.905、35.218,P<0.01)。结论Ferritin对MPP+诱导的MES23.5细胞损伤具有保护作用,能够拮抗MPP+诱导的细胞存活率和△Ψm的下降。

Objective To investigate the effect of exogenous ferritin on MES23.5cell damage induced by 1-methyl-4-phenylpyridinium(MPP+).Methods Methyl thiazolyl tetrazolium colorimetry was used to screen out the optimal concentration of MPP+for modeling.MES23.5dopaminergic neural cells were treated with MPP+,ferritin,or ferritin+MPP+.CCK8assay was used to measure cell viability,and flow cytometry was used to measure mitochondrial transmembrane potential(ΔΨm).Results Compared with the control group,the MPP+treatment group had significant reductions in cell viability andΔΨm of MES23.5cells(F=143.044and 70.924,P<0.01).Ferritin pretreatment for 4hours significantly inhibited the reductions in cell viability andΔΨm induced by MPP+(F=51.905and 35.218,P<0.01).Conclusion Ferritin exerts a protective effect against MPP+-induced damage in MES23.5cells and can antagonize the reductions in cell viability andΔΨm induced by MPP+.