低氧胁迫对凡纳滨对虾血蓝蛋白、自由氨基酸含量和体内Cu^(2+)转运的影响

The Influence of Hypoxia on the Contents of Hemocyanin and Free Amino Acids and the Transport of Cu^(2+) of Litopenaeus vannamei

研究了凡纳滨对虾(Litopenaeus vannamei)在溶解氧变化下血蓝蛋白、自由氨基酸含量和体内Cu2+转运的影响。结果表明:低氧胁迫对凡纳滨对虾血蓝蛋白含量、血浆和肝胰脏中Cu2+含量、血淋巴中总自由氨基酸含量和各种氨基酸含量的影响显著(P<0.05)。凡纳滨对虾血蓝蛋白含量在溶解氧变化36h内呈峰值变化,在12h时达最高值,在36~48h时低于对照组水平,且变化平稳,至72h时恢复至对照组水平;血浆中Cu2+含量先升后降,分别于6h、48h时达到最大值和最小值;而肝胰脏中Cu2+含量于6h后开始明显降低,12h时达到最小值,之后逐渐上升,于48h达到最大值并趋于稳定,稳定后Cu2+含量显著高于对照组(P<0.05);3.0mg/L溶解氧处理组血淋巴中总自由氨基酸含量和各种氨基酸含量均在48h内呈峰值变化,在24h时达到最大值,于48h时恢复至对照组水平;1.5mg/L溶解氧处理组血淋巴中总自由氨基酸含量和各种氨基酸含量在72h内呈峰值变化,均于24h时达到最大值,之后逐渐下降,至72h时仍显著高于对照组(P<0.05)。综上所述,在低氧胁迫下,凡纳滨对虾通过改变组织中自由氨基酸及Cu2+含量,迅速启动血蓝蛋白合成机制,调节血蓝蛋白含量以适应低溶解氧环境,在对虾体内环境稳定方面具有重要作用。

This study was carried out in order to evaluate the effect of hypoxia on the contents of hemocyanin and free amino acids and the transport of Cu^2＋of white shrimp（Litopenaeus vannamei）,The results showed that the hypoxia had significant effects on the contents of hemocyanin and free amino acids and the transport of Cu^2＋ of L.vannamei（P〈0.05）.The hemocyanin content raised first and then decreased with a peak appeared at 12 hin first 36 h.After that,it decreased further to a level lower than that of the control from 36 to 48h,and finally returned to that of the control.The Cu^2＋content in plasma raised first,reaching the maximum at 6hand then tdecreased,touching the minimum at 48 h.The Cu^2＋content in hepatopancreas decreased after 6h,reaching the minimum in 12 hand maxizing in 48 h and stabilizing gradually then.The free amino acid content in hemolymph in 3.0mg/L DO group showed a most obvious change in 48 hand reaching the maximum at 24 h,then returned to that of the control at 48 h.The free amino acid content in hemolymph in 1.5mg/L DO group also showed a most obvious change in 72 h,reaching the maximum at 24 h,then decreased,but was always higher than that of control.Overall,as a multifunctional protein in shrimp hemolymph,hemocyanin plays an important role in the shrimp homeostasis.