辛弗林对大鼠离体小肠收缩性的调节特征

The effect of synephrine on contractility of rat＇s isolated intestine

目的考察不同Ca^2＋浓度对大鼠离体小肠收缩性的影响；选择最佳Ca^2＋浓度，探讨在该条件下辛弗林对离体肠收缩性的影响。方法本研究以大鼠的离体小肠为模型，通过不同浓度的Ca^2＋克氏液，使小肠处于不同收缩状态；考察在不同ca2＋浓度条件下，辛弗林对离体小肠的收缩的影响。结果通过辛弗林对不同浓度的Ca^2＋条件下的作用比较，结果提示在5mmol／L的Ca^2＋隋况下，加入辛弗林后，肠平滑肌收缩幅度降低最明显。结论结果提示5mmol／LCa^2＋的浓度是在本实验条件下，用于研究对肠道平滑肌收缩具有抑制作用药物的最佳条件。

Objective To investigate the effect of Ca^2＋ concentration on contractility of rat＇s isolated intestine, and the effect of synephrine on contractility of rat＇s isolated intestine under the most suitable Ca^2＋ concentration. Methods Isolated rat intestine was selected in the assay to test the change- extent of contractile amplitude using Krebs＇ solution in different Ca^2＋ concentration, observed the effect of synephrine on contractility of isolated rat intestine in different Ca^2＋ concentration. Result The contractile amplitude decreased most significantly after adding synephrine when the concentration of Ca^2＋ was 5 mmol/L. Conclusion 5 mmol/L Ca^2＋ is the most suitable concentration to study inhibitory effect of a drug on isolated rat intestine in the corresponding assay condition.