分次少量取样对24小时尿标本检测结果的影响

Effect of small quantity sampling by several times on testing results of 24-hour urine sample

目的观察分次少量取样对24小时尿标本检测结果的影响，探讨一种简单科学的24小时尿标本留取方法。方法采用两种方法对67例患者的24小时尿标本进行采样，进行自身对照，测定其尿糖、尿蛋白、内生肌酐清除率、尿电解质，并对67例患者家属进行满意度调查。结果两种方法采样的标本检测尿糖（UG）、尿蛋白（UP）、内生肌酐清除率（Cer）及尿电解质（K、Na、C1），差异无统计学意义（P〉0.05）。患者家属对分次少量留取法满意度为98．5％（66／67），明显高于传统方法的82.1％（55／67），差异有统计学意义（P〈0.01）。结论分次少量取样对24小时尿标本检测结果无影响，可以替代传统的留取方法，值得推广应用。

Objective To observe the effect of small quantity sampling by several times on testing results of 24-hour urine sample, and explore a simple and scientific method for collecting 24-hour urine sample. Methods Collected 24-hour urine sample of 67 patients with two different methods. Tested urine glucose, urine protein, creatinine clearance rate （Ccr） and urine electrolyte. Recorded patients＇ families＇ satisfaction. Results There were no statistically significant differences in urine glucose, urine protein, Ccr and urine electrolyte （K, Na, C1） between two groups of samples （P〉0.05）. But the satisfaction of patients＇ families on small quantity sampling by several times was 98.5%, significantly higher than 82.1% on the traditional method, with statistically significant difference （P〈0.01）. Conclusion Small quantity sampling by several times has no significant effect on testing results of 24-hour urine sample, which can replace the traditional method, worthy of clinical promotion.