木薯MeASR基因克隆及表达分析

Clone and Expression of MeASR Gene in Cassava

脱落酸-胁迫-成熟诱导蛋白(Abscisic acid-stress-ripening,ASR)在植物对非生物逆境胁迫的应答过程中发挥着重要作用。利用PCR技术从木薯中克隆了第一个ASR基因Me ASR,序列分析表明该基因开放阅读框(ORF)330 bp,编码109个氨基酸。多序列比对和进化树分析表明该基因所编码的蛋白具有ASR家族蛋白的保守结构域,与番茄ASR家族蛋白Sl ASR4具有较近的亲缘关系。亚细胞定位分析表明Me ASR定位在细胞核,实时荧光定量PCR分析表明该基因的表达显著受渗透胁迫和ABA诱导。结果表明,Me ASR可能作为转录因子参与木薯对干旱逆境胁迫应答及ABA信号调节。

Abscisic acid-stress-ripening induced protein（ASR）plays an important role in response to abiotic stresses. In the present study we isolated a first ASR gene designated Me ASR from cassava. Sequence analysis showed that the open reading frame of Me ASR gene contained 330 bp, encoding 109 amino acids. Multiple sequence alignment and phylogenetic analysis indicated that Me ASR protein contained the conserved domains as ASR family had, and had a close genetic relationship with Sl ASR4 of tomato. Subcellular location assay showed that Me ASR protein was localized in nucleus. Real-time quantitative PCR assay revealed that expression of Me ASR was induced by osmotic stress and ABA treatments. These results suggested that Me ASR might function as a transcription factor to involve in response to drought stress and ABAsignal regulation in cassava.