被动吸烟孕妇胎儿GSTM1、GSTT1及CYP1A1基因多态性对胎儿出生畸形的影响

The impact of the GSTM1,GSTT1 and CYP1A1 gene polymorphism of Passive maternal fetal on birth deformity

目的探讨被动吸烟孕妇胎儿谷胱甘肽S转移酶M1(GSTM1)、谷胱苷肽硫转移酶T1(GSTT1)及细胞色素P450(CYP1A1)基因多态性对胎儿出生畸形的影响。方法 2009年1月至2014年12月选取在本院分娩、单胎活产出生畸形的新生儿85名为研究组,另选取同期在本院分娩的185例单胎活产正常新生儿为对照组,采用PCR技术检测两组新生儿GSTM1、GSTT1及CYP1A1基因型,运用多因素模型进行交换作用分析。结果研究组新生儿GSTM1、GSTT1基因缺失型及CYP1A1基因多态突变型频率显著高于对照组(P<0.05)。通过多因素模型进行交换作用分析可知,母亲孕期被动吸烟分别与GSTM1、GSTT1基因缺失型及CYP1A1基因多态突变型有正相加模型交互作用。交互效应超额相对危险度(RERI)分别为9.85、8.12、3.26,效应指数(S)分别为1.98、1.79、1.65。结论 GSTM1、GSTT1基因缺失型及CYP1A1基因多态突变型与孕妇孕期被动吸烟存在相加模型交互作用,可能与胎儿先天发育异常的发生有密切关系。

Objective：To investigate the impact of the glutathione S-transferase M1（GSTM1）,glutathione S transferase T1（GSTT1）and cytochrome P450（CYP1A1）gene polymorphism of Passive maternal fetal on birth deformity. Methods：85cases of single live births born deformed newborns named were selected as research groups and 185 cases in the hospital giving birth alone live births for normal newborn the were selected as control group from January 2013 to December 2014.The GSTM1,GSTT1 and CYP1A1 genotype of two groups was used with multivariate analysis model exchange interaction with PCR newborn. Results：The GSTM1,GSTT1 and CYP1A1 gene of deletion polymorphism mutant frequency was higher than control groups（P〈0.05）.The exchange effects by multivariate model analysis,the mother during pregnancy and passive smoking were GSTM1,GSTT1 and CYP1A1 gene deletion mutant gene polymorphisms have positive interactions additive model. Interaction excess relative risk（RERI）were 9.85,8.12,3.26,effect index（S）were 1.98,1.79,1.65. Conclusion：GSTM1,GSTT1 and CYP1A1 gene deletion mutant gene polymorphisms passive smoking during pregnancy and the presence of additive model interactions may occur closely associated with fetal congenital abnormalities.