精子DNA碎片率对辅助生殖结局的影响

Effect of sperm DNA fragmentation index on the outcome of in vitro fertilization-embryo transfer

目的探讨精子DNA碎片率与IVF-ET结局的相关性。方法对2013年1月至2014年4月因女方(≤35岁)输卵管因素到我中心行IVT-ET的男性患者进行回顾性分析,行精子DNA碎片率检查的共有112例,不同精子DNA碎片率按3分隔点分为3组,比较3组间的一般情况、精液常规参数、IVF受精情况及IVF-ET结局。结果 3组间精液参数无显著差异(P>0.05),C组IVF受精率最低(P<0.05),优胚率及移植优胚率最高(P<0.05),3组间比较卵裂率、胚胎着床率和临床妊娠率无差异(P>0.05)。结论 (1)精子DNA碎片率与精液常规各参数无相关性;(2)高精子DNA碎片率降低IVF受精率;(3)目前的胚胎评级并不足以预测胚胎结局,尚需进一步的研究。

Objective：To analyze the effect of Sperm DNA fragmentation index on the outcome of in vitro fertilizationembryo transfer（IVF-ET）.Methods：A retrospective study was conducted in 112 IVF-ET cycles of Institute of Reproduction,Luoyang Women ﹠ Children′s Health Care Hospital,among these cycles no women′s age were above 35 years old,and the cause of infertility was only fallopion tube factor. The cycles were divided into 3 groups（A,B,C）by different sperm DNA fragmentation index（DFI）,outcome of IVF-ET was compared among these groups.Results：The fertilization rate of IVF of C group was the lowest（P〈0.05）,and the good quality embryo rate of IVF of C group was the highest（P〈0.05）.No difference among the 3 groups of cleavage rate,embryo implantation rate and clinical pregnancy rate（P〉0.05）.Conclusions：High sperm DNA fragmentation index reduces the fertilization rate of IVF,the embryo grading standard is not enough to predict outcome,and more research is needed.