MicroRNA-154对大鼠肝星状细胞HSC-T6凋亡的影响

Effects of MicroRNA-154 on Apoptosis of Hepatic Stellate Cells

目的:探讨micro RNA-154(mi R-154)对大鼠肝星状细胞(HSC-T6)凋亡的影响。方法:用合成的mi R-154模拟物(mi R-154 mimics)和mi R-154抑制物(mi R-154 inhibitor)转染HSC-T6,以其阴性对照物(mimics NC、inhibitor NC)转染细胞作为阴性对照。采用CCK-8试剂检测mi R-154对HSC-T6增殖的影响;采用流式细胞术检测mi R-154对HSC-T6细胞周期和凋亡的影响。结果:mi R-154可以显著提高细胞增殖能力,降低细胞凋亡率(P<0.01);细胞内过表达mi R-154可使G0/G1期细胞比例显著下降,S期细胞比例显著升高(P<0.01);抑制细胞内mi R-154表达可显著提高细胞凋亡率,G0/G1期细胞比例显著升高,S期细胞比例显著下降(P<0.01)。结论:micro RNA-154可以促进肝星状细胞的增殖,抑制肝星状细胞的凋亡。

Objective：To investigate the effect of miR-154 on the apoptosis of hepatic stellate cells （HSC-T6）. Methods：Hepat-ic stellate cells （HSC-T6） were transfected with miR-154 mimics and miR-154 inhibitor, and the cells were trandfected with mimics NC and inhibitor NC as the negative control. The effects of miR-154 on the proliferation of HSC-T6 were detected at different time points by CCK-8. A flow cytometry with double staining of Annexin and PI was used to detect the cell cycle and apoptosis rate of HSC-T6. Results：The proliferation ability of the cells was increased,the apoptosis rate was decreased significantly, and the proportion of cells in G0/G1 phase were decreased, and those in G2/M phase were increased significantly after transfected with miR-154 mimics. The proliferation ability of the cells was decreased,the apoptosis rate was increased significantly, and the proportion of cells in G0/G1 phase were increased, and those in G2/M phase were decreased significantly after transfected with miR-154 inhibitor. Conclusion：MiR-154 can promote the proliferation of HSC-T6 and inhibit the apoptosis of HSC-T6.