芦笋不同部位多糖含量及抗肿瘤活性的研究

Study on Polysaccharide Content and Antitumor Activity in Different Parts of Asparagus

目的:研究芦笋食用部分与废弃部分中多糖含量及抗肿瘤活性的差异。方法:针对溶剂浓度、料液比、提取时间及提取温度4个因素的研究,采用正交试验确立芦笋两部分中多糖的最佳提取工艺后,对比两者多糖含量的差异,并观察其对MCF-7细胞的抗肿瘤活性差异。结果:分别用最佳工艺提取,芦笋食用部分与废弃部分中多糖含量比较,差异有统计学意义(P<0.05),将两部分精制多糖加入MCF-7细胞中,抗肿瘤活性差异无统计学意义(P>0.05)。结论:芦笋食用部分与废弃部分中多糖含量有显著差异,但两者对MCF-7细胞的抗肿瘤活性未有显著差异。

Objective：To study the content of polysaccharide and its antitumor activity in the edible part and the disused part of asparagus. Methods：A orthogonal design of 4 factors and 3 levels was applied to optimize the solvent concentration, solid-liquid ratio, extraction duration and temperature that influencing the extraction efficiency. The difference in the polysaccharides content between the two parts was compared, and the difference in the antitumor activity against MCF-7 cells was observed with SPSS software. Results：The optimum extraction technology was used. The difference in the polysaccharide content between the edible part and disused part of asparagus was significant （P〈0. 05）. The antitumor activity of the two refined polysaccharides against MCF-7 cells was without nota-ble difference （P〉0. 05）. Conclusion：There is significant difference in the content of polysaccharide in the edible part and the dis-used part of asparagus, however, there is no significant difference in the antitumor activity in the two parts of asparagus.