摘要
目的：建立柱前衍生化RP-HPLC法测定夜宁颗粒中二苯乙烯苷含量的方法。方法：采用丹酰氯作为衍生化试剂,色谱柱为Wondasil-C8色谱柱（250 mm x4.6 mm,5μm）,以（30 mM的乙酸铵用磷酸调节pH至3.5）-乙腈（78∶22）为流动相,柱温为30℃,流速为1.0 ml·min-1,荧光检测器检测（激发波长为370 nm,发射波长为560 nm）,进样量为20μl。结果：制剂中其他成分无干扰,二苯乙烯苷在0.071-27.280 mg·ml-1范围内线性关系良好,r=0.9999,平均回收率为99.04%,RSD为0.1%（n=9）。结论：所用方法简便快捷,灵敏度高,准确性好,可作为夜宁颗粒质量控制方法的依据。
Objective：To establish a RP-HPLC method with pre-column derivation for determining the content of stilbene glyco-sides in Yening granules. Methods：DNS-Cl was used as the derivation reagent, a Wondasil-C8 column （250 mm x 4. 6 mm, 5 μm） was used as the stationary phase and 30mM ammonium phosphatere gulation （pH 3. 5）-acetonitrile （78∶22） was applied as the mobile phase, a fluorescence detector （λex=370 nm,λem=560 nm） was used, the flow rate was 1. 0 ml·ml-1 , the column temperature was 30℃ and the injection volume was 20 μl. Results： There was no interference from the other substances in the preparation. The linear range of the two stilbene glycosides was 0.071-27.280 mg·ml-1（r=0.999 9）. The average recovery was 99.04%（RSD=0. 1%, n= 9）. Conclusion：The method is simple, quick, sensitive and accurate, which can be used for the quality control of Ye-ning granules.
出处
《中国药师》
CAS
2015年第11期2008-2010,共3页
China Pharmacist
