京尼平抑制解偶联蛋白2对激素非依赖性前列腺癌细胞能量代谢的影响

Inhibitory effect of Genipin on uncoupling protein-2 and energy metabolism of androgen-independent prostate cancer cells

目的:探讨京尼平对线粒体解偶联蛋白2(UCP-2)的抑制作用是否参与到激素非依赖性前列腺癌细胞(PC-3)的能量代谢中。方法:培养PC-3细胞并分别使用40、80、160μmol/L京尼平干预48 h,MTT法检测各组细胞的增殖情况;RT-PCR检测细胞内UCP-2 mRNA的表达水平;可见分光光度法测定细胞内丙酮酸含量和线粒体琥珀酸脱氢酶活性。结果:随着京尼平浓度的升高,PC-3细胞的增殖活性、细胞内UCP-2 mRNA的表达水平、细胞内丙酮酸含量和线粒体琥珀酸脱氢酶活性均逐渐下降,即随着京尼平对UCP-2抑制的增强,细胞增殖活性逐渐降低,细胞内丙酮酸含量和线粒体琥珀酸脱氢酶活性亦随之降低。结论:京尼平可能通过降低细胞内丙酮酸含量和线粒体琥珀酸脱氢酶活性的方式参与激素非依赖性前列腺癌细胞的能量代谢中,参与方式可能与抑制UCP-2有关。

Objective ： To explore whether the inhibitory effect of Genipin on uncoupling protein-2 （ UCP-2 ） in mitochondria is involved in energy metabolism of androgen-independent PC3 prostate cancer cells. Methods ： PC3 prostate cancer cells were cultured and treated with Genipin at the concentrations of 40, 80, and 160 μmol/L for 48 hours. Then the proliferation of the cells was detected by MTT assay, the expression of UCP-2 mRNA determined by RT-PCR, and the content of intracellular pyruvic acid （PA） and the activity of suceinate dehydrogenase （SDH） in the mitochondria measured by visible spectrophotometry. Results ： With the increased concentration of Genipin, the proliferative activity of the PC-3 cells, the expression level of UCP-2 mRNA, the content of intraeellular PA and the activity of SDH in the cells were all decreased, namely, with the enhanced inhibitory effect of Genipin on UCP-2, a trend of reduction was observed in the proliferation of the cells, intraeellular PA content, and SDH activity in the mitochondria. Conclusion ： Genipin is involved in the energy metabolism of androgen-independent PC3 prostate cancer cells by reducing the content of intracellular PA and the activity of SDH in the mitochondria, which may be associated with its inhibitory effect on UCP-2.