应用激光从完全不动精子中筛选存活精子行ICSI后获临床妊娠:附2例报告

Successful pregnancy following laser-assisted selection of viable but immotile spermatozoa for intracytoplasmic sperm injection: A report of 2 cases

目的:探讨应用激光筛选存活精子的可行性及临床应用价值。方法:对2例完全不动的精子(1例睾丸穿刺取精和1例射出精液),用激光射击精子尾部,选择激光射击后卷尾的精子用于卵细胞胞质内单精子注射(ICSI)。结果:2例患者用激光选择的精子行ICSI后,平均受精率达88.89%,新鲜周期移植后,两者均获得了临床妊娠。结论:激光是筛选存活精子的有效方法,临床应用可获得理想的妊娠结局。

Objective： To investigate the feasibility and clinical application value of selecting viable spermatozoa by noncontact diode laser. Methods ： We obtained immotile spermatozoa from 2 infertile men with obstructive azoospermia or severe asthenospermia and selected viable spermatozoa using a single laser shot at the sperm tail. Those that responded to the laser shot by a curling reaction of the tail were regarded as presumably viable and used for intracytoplasmie sperm injection （ICSI）. Results： The mean fertilization rate was 88.89% after ICSI with the laser-selected viable spermatozoa. Both of the embryo transfers resulted in a single pregnancy. Conclusion ： Noncontact diode laser is a useful alternative for the assessment of sperm viability, which may help to achieve successful pregnancy.