硒化纹党参多糖和其抗A549细胞的活性

Selenylation of polysaccharides from Codonopsis pilosula and its inhibition against A549 cells

目的合成硒化纹党参多糖并探讨其抗人肺腺癌细胞A549的活性。方法以HNO3-Na2Se O3方法硒化纹党参多糖,在单因素试验的基础上,以反应时间、反应温度、纹党参多糖与亚硒酸钠投料比为三因素进行L9(34)正交试验设计,选择硒化纹党参多糖含硒量、产率以及对A549细胞生长的抑制率作为考察指标,优选最佳硒化工艺。结果纹党参多糖的最佳硒化条件为反应时间5 h,反应温度60℃,投料比1∶1。在此条件下,硒化纹党参多糖中含硒量可达1.07 mg/g(RSD为3.7%),产率可达50.3%(RSD为2.5%),对A549细胞的抑制率可达到49.36%(RSD为2.8%)。结论硒化纹党参多糖可以作为抗肿瘤候选药物。

AIM To synthesize polysaccharides from Codonopsis pilosula selenide（ PCPs） and to explore its anti-tumor activity against human lung adenocarcinoma A549 cells. METHODS PCPs were synthesized by nitric acid-sodium selenide. Based on the single factor experiment,reactive parameters（ reaction time,reaction temperature and the ratio of polysaccharide to Na2 Se O3） were investigated and optimized by L9（ 3^4） orthogonal experimental design in terms of the selenite content,yield and the agent＇s inhibitory rate on A549 cells. RESULTS 5 h of reaction time,60 ℃ of reaction temperature and 1 ∶ 1 ratio of polysaccharide to Na2 Se O3were the optimal selenylation conditions. Under the optimal conditions,the mean selenium content,yield and inhibitory rate on tumor cell of prepared PCPs reached 1. 07 mg / g（ RSD = 3. 7%）,50. 3%（ RSD = 2. 5%） and 49. 36%（ RSD = 2. 8%）,respectively. CONCLUSION PCPs may be a promising antitumor agent.