清胰颗粒对重症急性胰腺炎大鼠肝、肾组织HMGB1表达的影响

Effect of Qingyi Granule on HMGB1 Expression in Liver and Renal Tissues of Severe Acute Pancreatitis Rats

目的探讨清胰颗粒(Qingyi Granule,QYG)对重症急性胰腺炎(severe acute pancreatitis,SAP)大鼠肝、肾组织中高迁移率族通道蛋白1(high mobility group box-1,HMGB1)表达的影响。方法54只SD大鼠按随机数字表分为假手术(sham operation,SO)组、SAP组和QYG组;SAP动物模型采用5%牛黄胆酸钠(sodium taurocholate,STC)诱导;苏木精和伊红(hematoxylin and eosin,HE)染色评价3组大鼠肝、肾病理损伤,ELISA法测血清淀粉酶(amylase,AMS)、丙二醛(MDA)、白细胞介素-1(IL-1)和HMGB1水平,免疫组化检测肝、肾组织中HMGB1蛋白表达;逆转录-聚合酶链式反应(RTPCR)检测肝、肾组织HMGB1 mRNA表达。结果 SAP组大鼠肝、肾组织病理评分、血清AMS、MDA、IL-1和HMGB1水平和肝、肾组织HMGB1蛋白及mRNA表达均较SO组明显升高(P<0.05,P<0.01);QYG能有效下调上述各指标血清水平和肝、肾组织HMGB1蛋白及mRNA表达量,治疗72 h疗效最显著(P<0.05,P<0.01),并呈时效依赖性。结论 HMGB1参与SAP并发肝、肾组织损伤过程,QYG可有效抑制HMGB1表达,从而减轻SAP时肝、肾组织损伤。

Objective To explore the effect of Qingyi Granule（QYG） on high mobility group box-1（HMGB1） expressions in liver and renal tissues of severe acute pancreatitis（SAP） rats.Methods Fifty-four Sprague-Dawley（SD） rats were divided into the sham-operation（SO） group,the SAP group,and the QYG group according to random digits table.Rats in the SAP group were induced by injecting 5%sodium taurocholate（STC）.Liver and renal pathological changes were observed by HE staining.Serum contents of amylase（AMS）,MDA,IL-1,and HMGB1 were detected by ELISA.HMGB1 protein expressions in liver and renal tissues were tested by immunohistochemistry.HMGB1 mRNA expressions in liver and renal tissues were detected by reversed transcription PCR.Results The pathological scores,serum levels of AMS,MDA,IL-1 and HMGB1,and protein and mRNA HMGB1 expressions in liver and renal tissues were increased more obviously in the SAP group than in the SO group（P 0.05,P 0.01）.All of them could be down-regulated by QYG intervention,with the most significant effect seen at 72 h（P 0.05,P 0.01） in a time-effect relationship.Conclusions HMGB1 participated in SAP complicated liver and renal injuries.QYG could effectively inhibit HMGB1 expressions,thereby attenuating SAP complicated liver and renal injuries.