红锥4种叶部病害病原鉴定

Identification of Pathogen of Four Leaf Diseases on Castanopsis hystrix

为调查广西地区红锥(Castanopsis hystrix)叶部真菌性病害的种类,本研究从广西南宁市良凤江森林公园、博白林科所、博白林场和高峰林场4个地区采集红锥叶部病害样本,使用常规组织块分离法获取其致病菌,结合形态学观察和r DNA-ITS序列分析进行病害鉴定。结果表明:红锥叶部发生的主要病害有4种,分别为赤斑病(Pilidella granati)、褐斑病(Pestalotiopsis pauciseta)、炭疽病(Colletotrichum gloeosporioides)、藻斑病(Cephaleuros virescens Kunze);其中,前3种病害为在红锥上新发生的病害。

For investigating the species of fungal pathogen of leaf diseases on diseased samples of C. hystrix were collected from seedling garden in Nanning Castanopsis hystrix , the Liangfengjiang National Forest Park of Guangxi Province, the research institution of forestry in Bobai County, the center of for- estry in Bobai County, the center of forestry in Gaofeng. By means of combining morphological charac- teristics with molecular biological techniques, there were four species of leaf diseases on C. hystrix, which were red spot （ Pilidella granati）, brown spot （ Pestalotiopsis pauciseta）, anthracnose （ Colleto- trichum gloeosporioides）, algal spot （ Cephaleuros virescens Kunze）. Among them, the former three dis- eases are new diseases on C. hystrix.