抗黑条矮缩病水稻品种资源的筛选与鉴定

Screening and Evaluation of Rice Resources Resistance to Rice Black-Streaked Dwarf Virus Disease

近年来,水稻黑条矮缩病严重影响我国水稻生产,培育高抗黑条矮缩病的水稻新品种迫在眉睫。获得抗性好的水稻资源是育种的基础。本研究通过对保存的2000份水稻地方品种连续2年设置2个试验点的黑条矮缩病抗性鉴定,初步筛选出连续2年没有发病的资源38份。对这38份中农艺性状较好的6份粳稻资源进行人工室内接虫鉴定,获得高抗水稻黑条矮缩病的水稻资源1份。与感病对照相比,水稻黑条矮缩病毒(RBSDV,rice black-streaked dwarf virus)在抗病品种体内的表达量下降71.5倍,表明抗病品种对RBSDV在体内的复制有明显的抑制作用。本研究为水稻黑条矮缩病抗病育种和抗性基因的定位、克隆奠定了材料基础。

Rice black-streaked dwarf virus （RBSDV） disease has seriously affected rice production in China in recent years, giving rise to an imperative of breeding rice new varieties with high resistance to RBSDV. Rice resources highly resistant to RBSDV play key roles in breeding procedure. In this study,2000 local varieties were evaluated on the resistance to RBSDV for two consecutive years. 38 varieties without disease symptoms were primarily screened, of which 6 japonica varieties with preferred agronomic traits were selected to perform artificial inoculation identification. One variety highly resistance to RBSDV was obtained ultimately. The expression of RBSDV decreased 71.5 times compared to the sensitive control, indicating a notable inhibition of RBSDV replication in the disease-resistant variety. This study laid the material foundation for RBSDV resistance breeding procedure and cloning of resistance genes.