云南甘蔗常用亲本资源遗传多样性的SSR分析

Genetic Diversity Analysis of Yunnan Commonly-used Parents by Using SSR Marker

以63份云南育种机构常用的甘蔗亲本资源为研究对象,10份甘蔗原始亲本种为外群体,使用30对多态性较高、在甘蔗遗传连锁图谱上分布较为广泛的Genomic-SSR引物对云南甘蔗常用亲本资源开展遗传多样性评价。结果表明:云南甘蔗常用亲本在30个SSR位点上表现出丰富的多态性,共获得363个扩增条带,其中多态性条带数为352个,平均多态性条带比例和多态性信息量分别为96.97%和0.9441;在Jaccard相似性系数方面,云南甘蔗常用亲本材料之间的相似性系数范围在0.2804~0.7329之间,平均为0.4309,表现出较大的遗传差异,其中来自大陆地区的亲本遗传差异最大,其次为来自国外地区的亲本;UPGMA聚类分析将所有亲本材料分为一大一小两个类群,而大类群又可分为2个亚类群,中国大陆地区一些既是主栽品种又是亲本的材料表现出较近的亲缘关系,而引自国外的大部分亲本和一些老的国内自育亲本也表现出较近的亲缘关系。以上结果将为上述亲本资源的高效利用奠定良好的基础,并为全国育种机构在亲本选择和组合配置上提供重要参考。

Sugarcane parents are important genetic resources for genetic breeding and innovation of sugarcane. In order to learn the genetic diversity of Yunnan sugarcane commonly-used parents, about 63 clones were screened by using 30 pairs of high polymorphic Genomic-SSR primer with 10 origin species of sugarcane as outgroup. The results showed that total 363 bands were obtained by these SSR primers,of which 353 were polymorphic bands. High Percentage of polymorphic bands and polymorphism information content（96.97% and 0. 9441 ）indicated that Yunnan sugarcane commonly-used parents possessed rich g,.~net^c diverskyo The analysis of Jaccard similar coefficient of all clones illustrated big genetic differences appeared among these parents with a low average similar coefficient of 0. 4309 （range between 0. 2804 and 0. 7329）. These parents from main land of China exhibited the biggest genetic differences, followed by these parents from foreign countries. By using UPGMA cluster method, all parents could be assigned into two groups of different sizes. Moreover, the big group containing most of parents also could be segregated into two subgroups. Based on the results of clustering, some parents from main land of China, which were not only excellent parent but also main euhivars, performed a close genetic relationship. And this relationship was also found between these parents from foreign countries and some old parents from main land of China. Finally, these results mentioned above would improve the exploring efficient of Yunnan sugarcane commonly-used parents and pro-vide informative direction for screening parent and making cross combination in breeding and innovation programs.