白藜芦醇对大鼠急性酒精性肝损伤的保护作用

Protective effects of resveratrol on alcohol-induced acute hepatic injury in rats

目的建立大鼠的急性酒精性肝损伤模型,研究体内白藜芦醇对急性酒精性肝损伤的保护作用,同时初步探讨其作用机制。方法采用完全随机法将SD大鼠分为6组:空白对照组,模型组,白藜芦醇高(100 mg/kg)、中(50 mg/kg)、低(25 mg/kg)剂量组及阳性对照组。除空白组外其他组大鼠每日以55度红星二锅头灌胃,每次7.5 ml/kg,2次/日,空白组大鼠则每日以等容剂的生理盐水灌胃。另外,白藜芦醇高、中、低剂量组及阳性对照组大鼠每日以相应药物灌胃给药,1次/日;空白组及模型组大鼠则以等容剂的1%的羧甲基纤维素钠灌胃。连续灌胃7 d。第8天取大鼠血液及肝脏,检测血清及肝组织匀浆中的谷丙转氨酶(ALT)、谷草转氨酶(AST)、超氧化物歧化酶(SOD)及丙二醛(MDA)。同时检测血清中白介素-1β(IL-1β)和肿瘤坏死因子-α(TNF-α)的水平,另取部分肝脏组织做病理切片。结果与空白对照组比较,模型组的血清及肝匀浆中的ALT、AST活力及MDA含量显著升高,且SOD活力显著降低;白藜芦醇高、中、低剂量组较模型组ALT、AST活力及MDA含量显著降低,SOD活力显著提高;白藜芦醇显著降低急性酒精性肝损伤大鼠血清中IL-1β和TNF-α水平的升高。病理学检查显示白藜芦醇治疗组能改善模型组大鼠细胞水样变性及脂肪样变性。结论白藜芦醇对大鼠急性酒精性肝损伤有保护作用,其机制可能与清除氧自由基、抗脂质过氧化损伤、抑制炎症反应水平有关。

This study performed to establish a model of acute alcohol-induced liver injury in rats and to study the protective effect of resveratrol on the acute alcoholic hepatic injury in rats and its mechanism. The SD rats were divided into six groups randomly： negative control group, model group, high-dose group（resveratrol, 100 mg/kg）,middle-dose group（resveratrol, 50 mg/kg）, low-dose group（resveratrol, 25 mg/kg） and positive control group. The model rats were orally administrated with 55% alcohol, 7.5 ml/kg per time, 2 times a day, while the negative control group was administrated with saline; the high-dose, middle-dose and low-dose of resveratrol and positive control group were orally administrated with corresponding drug every day, while the negative control group and model group were orally administrated with the 1% sodium carboxymethyl cellulose. All rats were treated for 7 days, and at the8 thday of the treatment, the blood and liver homogenate of the rats were collected to measure alanine amino transaminase（ALT）, aspartate amino transaminase（AST）, superoxide dismutase（SOD）, and malondialdehyde（MDA）; the levels of IL-1βand TNF-α in serum were measured; and another part of the remaining liver tissues were taken for pathological section.Compared with negative control group, the MDA level and the ALT, AST activity of the model group significantly increased, but SOD activity significantly decreased. While the MDA level and the ALT, AST activity of the treatment group were much lower but SOD activity is much higher as compared with model group. Compared with model group, the levels of IL-1β and TNF-α were significantly lowered by resveratrol. Pathological examination exhibited that the hydropic and lipoid cellular degeneration of rats in model group can be reduced significantly by resveratrol treatment. In conclusion, resveratrol has protective effect against acute alcoholic hepatic injury in rat, and the mechanism may associated with free radical scavenging activity, anti-lipid peroxidation injury, and inflammatory reaction suppression.