S-甲酸谷胱甘肽水解酶与类风湿性关节炎的相关性

The correlation between S-formylglutathione hydrolase and rheumatoid arthritis

目的检测类风湿性关节炎(rheumatoid arthritis,RA)早期和晚期患者血清中S-甲酸谷胱甘肽水解酶(Sformylglutathione hydrolase,Es D)水平,探讨Es D与RA的相关性及其诊断意义。方法以患者血清和正常人血清,采用二维凝胶电泳免疫印迹的比较寻找差异蛋白点,质谱测序和Western blot证明患者血清Es D在患者高表达、在正常人则检测不到。在此基础上,分别对早期、晚期、非RA患者(其它自身免疫性疾病)和正常人20例、40例、30例、40例血清通过ELISA的方法检测Es D水平,分析血清Es D水平与RA的相关性。结果 RA血清中的Es D水平明显高于非RA患者(P<0.001)和正常人(P<0.001),在早期和晚期RA中的敏感性分别为75%和72.5%,特异性为95%;进一步比较与RA现有诊断指标的相关性,发现Es D血清水平与ESR、CCP、DAS28评分具有显著相关性(P<0.05)而与RF无显著相关(P>0.05);与临床皮质激素治疗呈负相关。结论提示Es D血清水平对RA可能具有早期诊断价值;与激素治疗负相关说明该指标反映了疾病活动度;其在RA疾病发生及进展中的作用机理有待于进一步研究。

This study aimed to detect the serum level of S-formylglutathione hydrolase（Es D） in patients with early or late RA and probe into the relevance between Es D and RA, as well as to probe into its values in RA diagnosis. Two dimensional electrophoresis（2-DE） was utilized to seek differential protein points in the serum samples of patients and normal people, and then mass spectrometry and Western blot confirmed that the Es D was obviously higher expressed in the serum samples of RA patients but did not detectable in the serum samples of normal group. Based on this result, ELISA was used to detect the Es D level in serum of 20 early RA patients, 40 late RA patients, 30 non- RA patients（other auto-immune diseases） and 40 normal individuals, in order to analyze the relevance of serum Es D level with RA. Data showed that Es D level in RA serum was obviously higher than that of nonRA（P〈0.001） and normal groups（P〈0.001）; the sensitivity of serum Es D level in early RA and late RA were 75% and72.5%, and the specificity was 95%. Furthermore, the level of serum Es D has obvious relevance with ESR, CCP and DAS28 score（P〉0.05）, has no relevance with RF（P0.05）, and has negative relevance with clinical corticosteroid therapy.In conclusion, this study indicated that serum Es D level perhaps has early diagnosis value for RA. The negative relevance of serum Es D level with corticosteroid therapy suggests that this marker reflects the disease activity; the role of Es D in the RA disease occurrence and development need more research.