可见分光光度法对3种细菌计数的研究

Study on Bacteria Counts by Vis Spectrophotometry

目的：建立枯草芽孢杆菌、短小芽孢杆菌、藤黄微球菌的快速计数方法。方法：采用可见分光光度法进行细菌计数方法研究，考察菌液浓度与吸光度之间的关系。结果：枯草芽孢杆菌、短小芽孢杆菌、藤黄微球菌菌液的吸光度与其菌液浓度对应在0.68×10^8～10.88×10^8、0.80×10^8～12.80×10^8、0.89×10^8～14.30×10^8浓度范围内线性关系良好（r2分别为0.9999、0.9998、0.9998，n=5），相应菌落计数结果稳定。结论：可见分光光度法可用于3种细菌菌液的快速计数，可以提高药品微生物检定工作效率。

Objective：To establish rapid bacterial counting methods forBacillus subtilis, Bacillus pumilusand Micrococcus luteus.Methods： Vis spectrophotometry was employed for bacterial counts. The correlation between absorbance and concentration of bacteria solution was investigated.Results：The absorbance and the concentration of bacteria solution had good linear relationship （r2=0.9999, 0.9998, 0.9998,n=5） when the absorbance and of Bacillus subtilis solution,Bacillus pumilus solution andMicrococcus luteus solution were respectively between 0.68×10^8～10.88 ×10^8, 0.80×10^8～12.80×10^8, 0.89×10^8～14.30×10^8. The results of bacterial counts were stable. Conclusion：Vis spectrophotometry is suitable in the three kinds of bacteria counting, which can signiifcantly improve the working efifciency in medicine microbial tests.