摘要
目的:建立枯草芽孢杆菌、短小芽孢杆菌、藤黄微球菌的快速计数方法。方法:采用可见分光光度法进行细菌计数方法研究,考察菌液浓度与吸光度之间的关系。结果:枯草芽孢杆菌、短小芽孢杆菌、藤黄微球菌菌液的吸光度与其菌液浓度对应在0.68×10^8~10.88×10^8、0.80×10^8~12.80×10^8、0.89×10^8~14.30×10^8浓度范围内线性关系良好(r2分别为0.9999、0.9998、0.9998,n=5),相应菌落计数结果稳定。结论:可见分光光度法可用于3种细菌菌液的快速计数,可以提高药品微生物检定工作效率。
Objective:To establish rapid bacterial counting methods forBacillus subtilis, Bacillus pumilusand Micrococcus luteus.Methods: Vis spectrophotometry was employed for bacterial counts. The correlation between absorbance and concentration of bacteria solution was investigated.Results:The absorbance and the concentration of bacteria solution had good linear relationship (r2=0.9999, 0.9998, 0.9998,n=5) when the absorbance and of Bacillus subtilis solution,Bacillus pumilus solution andMicrococcus luteus solution were respectively between 0.68×10^8~10.88 ×10^8, 0.80×10^8~12.80×10^8, 0.89×10^8~14.30×10^8. The results of bacterial counts were stable. Conclusion:Vis spectrophotometry is suitable in the three kinds of bacteria counting, which can signiifcantly improve the working efifciency in medicine microbial tests.
出处
《中国药事》
CAS
2015年第10期1066-1068,共3页
Chinese Pharmaceutical Affairs