摘要
目的了解浙江省绍兴市男男性行为者(MSM)人群中HIV-1感染者的亚型类型和特征。方法分析2013年1月至2014年7月本地区新确证未经抗病毒治疗的50例MSM感染者样本,采用RT-PCR和nest-PCR方法扩增HIV-1的gag和pol全长基因区,成功扩增46份样品,构建系统进化树分析基因亚型。结果 46份样品中发现CRF01_AE、CRF07_BC和B三种亚型和1株AE、BC重组亚型,各占52.17%、39.13%、6.52%和2.17%。结论绍兴市HIV-1感染的MSM人群中主要流行的亚型为CRF01_AE和CRF07_BC,该人群感染率高,应加强监测。
Objective To understand the genotypes of HIV-1 circulating in HIV infected men who have sex with men（ MSM） in Shaoxing,Zhejiang province. Methods Fifty blood samples were collected from 50 newly-diagnosed HIV infected MSMwho received no antiviral therapy from 2013 to 2014,and the gag and pol gene fragments of HIV-1 were amplified with RT-PCR and nest-PCR. The gene phylogenetic tree was constructed to analyze the subtypes of HIV strains. Results A total of 46 plasma samples were successfully amplified and analyzed. Three HIV subtypes were detected,i. e. CRF01_ AE,CRF07_ BC and B,accounting for 52. 17%,39. 13% and 6. 52%. In addition,1 newrecombinant subtype was detected（ 2. 17%）. Conclusion CRF01_AE and CRF07_BC were the major HIV-1 subtypes detected among MSMin Shaoxing. It is necessary to strengthen the surveillance of HIV-1 subtype CRF01_ AE and CRF07_BC in MSM.
出处
《疾病监测》
CAS
2015年第10期841-845,共5页
Disease Surveillance
基金
绍兴市科技计划项目(No.2013B70065)~~
