肝细胞癌患者血清中Talin1的表达及其临床意义

Clinical significance of serum Talin1 detection for patients with hepatocellular carcinoma

目的检测肝细胞癌(HCC)患者血清Talin1的水平,评估其在HCC临床诊断中的价值。方法收集40例HCC患者、30例肝硬化(LC)患者、30例健康对照者(HC)的静脉血,分离血清,采用酶联免疫吸附试验(ELISA)检测血清中Talin1的水平,并与常规检测血清甲胎蛋白(AFP)的结果进行比较。应用受试者工作特征曲线分析Talin1相对于AFP在HCC中的诊断价值。结果HCC患者血清Talin1的水平明显高于LC和HC组(P<0.001)。在诊断HCC的敏感度、特异度、阳性预测值和阴性预测值等方面,Talin1的诊断精确性高于AFP。结论 Talin1是一种新型的诊断标志物,可以用于HCC的临床诊断,其敏感度和特异度高于传统的诊断标志物AFP。

Objective To detect the serum Talin1 levels and assess the diagnostic value of serum Talin1 for the diagnosis of hepatocellular carcinoma（ HCC）. Methods Talin1 was detected by enzyme linked immunosorbent assay（ ELISA） in serum samples from 100 subjects [including 40 with HCC,30 with liver cirrhosis（ LC） and 30 healthy controls（ HC） ]. The diagnostic value between alpha-fetoprotein（ AFP） and Talin1 for HCC was compared. Results Receiver operating characteristic curve analysis was used to create a predictive model for Talin1 relative to AFP in HCC diagnosis. Serum levels of Talin1 in HCC patients were significantly higher compared to the other groups（ P 0. 001）. The diagnostic accuracy of Talin1 was higher than that of AFP regarding sensitivity,specificity,positive predictive value and negative predictive value in diagnosis of HCC. Conclusions Talin1 is a new diagnostic marker for HCC,with a higher sensitivity and specificity compared to the traditional biomarker AFP.