hsa—miRNA-451靶基因预测及生物信息学分析

Prediction and bioinformatic analysis of hsa-miRNA-451 target genes

目的对hsa—miRNA-451进行靶基因预测及相关生物信息学分析。方法选择miRBase、miRanda、TargetScan、PicTar数据库或预测软件预测hsa—miRNA-451靶基因。对预测的hsa—miRNA-451靶基因的基因集合进行功能富集分析和信号转导通路富集分析。采用Fisher精确概率法计算P值，P〈0．05为显著性阈值。结果至少3个数据库或预测软件预测到hsa—miRNA-451靶基因的靶点18个。功能富集分析预测的结果显示，hsa—miRNA-451靶基因主要参与上皮细胞增生、发育以及调节酶活性相关生物过程（P〈0．05）。信号转导通路富集分析预测的结果显示，has—miRNA-451靶基因显著富集于转化生长因子-8信号通路、丝裂原活化蛋白激酶信号通路、表皮生长因子信号通路、Wnt信号通路、哺乳动物雷帕霉素靶蛋白信号通路等（P〈0．05）。结论hsa—miRNA451可调控与上皮细胞增生、发育相关的多个信号通路。

Objective To predict as well as bioinformatically analyze the target genes of has-miR-451. Methods miRBase, miranda, TargetScan and PicTar were used to predict the target genes of hsa-miRNA- 451. The functions of the target genes were demonstrated by Gene Ontology and pathway enrichment analysis. P〈0.05 was set as statistically significant. Results 18 target spots of hsa-miRNA-451 were predicted by 3 databases or prediction software at least. The functions of the target genes were enriched in proliferation and development of epithelial cells and regulation of kinase activity （P 〈 0.05）. Pathway analysis showed that transforming growth factor-beta signaling pathway, mitogen-activated protein kinase signaling pathway, epidermal growth factor signaling pathway, Wnt signaling pathway and mammalian target of rapamycin signaling pathway were significantly enriched （P〈0.05）. Conclusion hsa-miRNA-451 might be involved in various signaling pathways related to proliferation and development of epithelial cells.