石菖蒲大孔树脂不同洗脱部位高效液相图谱与抗抑郁效应的关联性研究

Correlation study on high performance liquid chromatography and antidepressant effect of different eluting fractions of acorus tatarinowii schott macroporous resin

目的研究石菖蒲大孔树脂不同洗脱部位高效液相图谱与抗抑郁效应的关联性。方法采用AB-8大孔树脂制备石菖蒲不同部位。采用梯度洗脱法建立石菖蒲原药材及不同树脂洗脱部位的高效液相特征图谱,并确认图谱中特征峰及对应特征峰之间的峰面积数据关系。运用小鼠悬尾实验(TST)、游泳实验(FST)等小鼠行为学实验,观察口服石菖蒲及不同树脂洗脱部位抗抑郁效应。结果石菖蒲原药材及不同AB-8树脂洗脱部位的特征图谱具有特征共有峰。石菖蒲大孔树脂90%及70%乙醇洗脱部分能显著缩短TST和FST。石菖蒲抗抑郁效应与特征峰具有关联性。结论石菖蒲具有抗抑郁作用,其主要抗抑郁效应来源于AB-8树脂70%及90%乙醇洗脱部位。石菖蒲液相特征图谱及相关峰数据关系可用于衡量石菖蒲的抗抑郁效应,为今后石菖蒲用于抗抑郁效应研究及开发提供依据。

Objective To research the correlation on high performance liquid chromatography（HPLC）and antidepressant effect of different elution fractions of acorus tatarinowii schott macroporous resin.Methods Prepared different fractions by using AB-8macroporous resin.Establish HPLC characteristic chromatograms of crude drugs and different elution fractions from AB-8acorus tatarinowii schott macroporous resin by using gradient elusion.Confirm characteristic peaks in chromatograms.despair models of male mice,including tails suspension test（TST）,forced swimming test（FST）were used to evaluate antidepressant effects by taking orally.Results HPLC chromatograms of crude drugs and different fraction of acorus tatarinowii schott showed that they had same characteristic peaks.Elution of 90% and 70% alcohol solution from AB-8macroporous resin can obviously reduce TST and FST time.The anti-depressant effect were associated with characteristic peaks on chromatogram.Conclusion Acorus tatarinowii schott has anti-depressant effect,and mainly from 70%and 90%alcohol solution eluted from AB-8macroporous resin.HPLC characteristic chromatography and data relationship between relative peaks can be used to judge the antidepressant effects of acorus tatarinowii schott,and give reference to research and product development.