DLC1和RhoA表达对乳腺癌淋巴道转移的影响

The effect of DLC1 and RhoA in lymphatic metastasis of breast cancer

目的探究肝癌缺失基因1(DLC1)与RhoA表达对乳腺癌淋巴道转移的影响。方法收集已发生淋巴结转移的乳腺癌病变组织标本25例,未发生淋巴结转移的乳腺癌病变组织标本30例,采用免疫组化(SP)法检测DLC1与RhoA在两组标本中的表达情况,应用统计学方法对实验结果进行分析。结果在发生转移和未发生转移的乳腺癌病变组织中DLC1阳性率分别为44.00%和73.33%(χ2=4.889,P=0.027),RhoA阳性率分别为92.00%和50.00%(χ2=11.264,P=0.001),spearman等级相关分析显示DLC1与RhoA在乳腺癌病变组织中的表达呈负相关(r=-0.454,P=0.000)。结论乳腺癌淋巴道转移与DLC1低表达、RhoA高表达密切相关。

Objective Through detect the expression of DLC1 and RhoA protein in breast cancer tissues,investigate the effect of DLC1 and RhoA in lymphatic metastasis of breast cancer.Methods Totally 25 breast cancer specimens that had occurred lymph node metastasis and 30 breast cancer specimens that had not occurred lymph node metastasis were collected,the expression of DLC1 and RhoA were tested by immunochemistry,and the experimental data were analyzed by statistical methods.Results The positive rate of DLC1 in the breast cancer specimens that had occurred lymph node metastasis was 44.00%,that had not occurred lymph node metastasis was 73.33%（χ2=4.889,P=0.027）,and the positive rates of RhoA in the two specimens were 92.00%and 50.00%（χ2=11.264,P=0.001）.Spearman rank correlation analysis showed the expression of DLC1 and RhoA had a negative correlation in breast cancer tissues（r=-0.454,P=0.000）.Conclusion The breast cancer lymphatic metastasis and is closely related to the low expression of DLC1 and the high expression of RhoA.