期刊文献+

胡杨无性系幼苗响应盐胁迫的miRNA表达差异研究 被引量:2

Salt-responsive MicroRNAs in Populus euphratica by Deep Sequencing
下载PDF
导出
摘要 植物mi RNA在调控基因表达、细胞周期、生物体发育、抗逆等方面起重要作用。为研究胡杨(Populus euphratica Oliv.)的耐盐机制,以1年生胡杨无性系幼苗为材料,构建具有空间代表性的盐胁迫胡杨c DNA文库,利用二代测序技术测定Na Cl胁迫下和正常培养条件下胡杨叶和根mi RNA表达情况。结果表明,不同的mi RNA之间表达量存在明显差异,表达丰度最高的mi RNA有mi R156、mi R157、mi R165、mi R166和mi R167等,合计占总表达量的90%以上。胡杨根部存在特异表达的mi RNA,在整个耐盐调控机制中发挥着生理调节、分子调控和信号传导等极为重要的作用。盐处理样品中发现大量响应盐胁迫的mi RNA,对这些转录因子进行靶基因预测和注释后,发现很多盐胁迫响应的mi RNA与NAC和SPL等重要转录因子家族相关,与前人的结论一致,另外还发现许多mi RNA的调控对象是ATP酶和激素响应因子。 MicroRNAs (miRNAs) have important regulatory functions in plant growth, development, cell cycle, gene expression regulation and response to abiotic stress. For studying the resistance of Populus euphratica to salt, we used 1-year-old poplar cutting clones to construct four small cDNA libraries from P. euphratica plantlets. We used the high-throughput sequencing to identify differentially expressed miRNAs treated with or without salt (300 mmol · L^-1 NaCl, 7 days). The different miRNA had significantly different expression. More than 90% expression were appeared in miR156, miR157, miR165, miR166, and miR167. The miRNA specific expressed in root played extremely important role in physiological, molecular regulation and signal transduction. Especially, many miRNAs were found in response to salt stress, and their target genes were predicted and annotated. These miRNAs were related to NAC and SPL transcription factor family, and they regulated ATP enzyme andhormone response factor.
出处 《植物研究》 CAS CSCD 北大核心 2015年第6期836-842,共7页 Bulletin of Botanical Research
基金 国家自然科学基金(31401138) 中央高校基本科研业务费专项资金项目(TD2012-04)
关键词 高通量测序 MIRNAS 胡杨 盐胁迫 high-throughput sequencing microRNA Populus euphratica salt stress
  • 相关文献

参考文献29

  • 1Bartel D P. MicroRNAs : genomics, biogenesis, mechanism, and function [ J ]. Ce11,2004,116 (2) :281 - 97.
  • 2Jones-Rhoades M W, Bartel D P, Bartel B, et al. MicroR- NAs and their regulatory roles in plants [ J ]. Annu Rev Plant Biol, 2006,57 : 19 - 53.
  • 3Lee R C,Feinbaum R L,Ambros V. The C. elegans hereto- chronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14 [ J ]. Cell, 1993,75 ( 5 ) : 843 - 854.
  • 4Griffiths-Jones S, Russell J Groeoek, Stijnvan van Dongen, et al. miRBase:microRNA sequences, targets and gene no- menclature [ J ]. Nucleic Acids Res, 2006,34 ( Database is- sue) :140 - 144.
  • 5Liu H H,Tian X,Li Y J,et al. Microarray-based analysis of stress-regulated microRNAs in Arabidopsis thaliana [ J ]. Rna,2008,14 ( 5 ) : 836 - 843.
  • 6Jia Xiaoyun, Wang Wangxia, Ren Ligang, et al. Differential and dynamic regulation of miR398 in response to ABA and salt stress in Populus tremula and Arabidopsis thaliana [ J]. Plant Mol Biol,2009,71 ( 1 ) :51 - 59.
  • 7Trindade I, Capitao C, Dalmay T, et al. miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula [ J ]. Planta, 2010, 231 ( 3 ) : 705 - 716.
  • 8Zhou Liguo, Liu Yunhua, Liu Zaochang, et al. Genome-wide identification and analysis of drought-responsive microR- NAs in Oryza sativa[ J]. J Exp Bot,2010,61 (15) :4157 - 4168.
  • 9Li B, Qin Y, Duan H, et al. Genome-wide characterization of new and drought stress responsive microRNAs in Popu- lus euphratica[J]. J Exp Bot,2011,62(11) :3765 -3779.
  • 10Wang T, Chen L, Zhao M, et al. Identification of drought- responsive microRNAs in Medicago tmncatala by genome- wide high-throughput sequencing [ J ]. BMC Genomics, 2011,15(12) :367.

二级参考文献65

  • 1陈玉玲,李建华,王学臣.蚕豆保卫细胞中钙调素的免疫电镜定位[J].植物生理学通讯,2003,39(6):609-612. 被引量:4
  • 2Yu Chang Shao-Liang Chen Wei-Lun Yin Rui-Gang Wang Yan-Feng Liu Yong Shi Yuan-Yuan Shen Yue Li Jie Jiang Yue Liu.Growth, Gas Exchange, Abscisic Acid, and Calmodulin Response to Salt Stress in Three Poplars[J].Journal of Integrative Plant Biology,2006,48(3):286-293. 被引量:8
  • 3李培旺,卢向阳,李昌珠,方俊,田云.植物microRNAs研究进展[J].遗传,2007,29(3):283-288. 被引量:7
  • 4Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell, 2009, 136(2): 215-233.
  • 5Lee RC, Feinbaum RL, Ambros V. The C. elegans hereto- chronic gene lin-4 encodes small RNAs with antisense complementarity to lin-14. Cell, 1993, 75(5): 843-854.
  • 6Cuperus JT, Fahlgren N, Carrington JC. Evolution and functional diversification of MIRNA genes. Plant Cell, 2011, 23(2): 431-442.
  • 7Axtell MJ, Bartel DE Antiquity of microRNAs and their targets in land plants. Plant Cell, 2005, 17(6): 1658-1673.
  • 8Zhang BH, Pan XP, Cannon CH, Cobb GP, Anderson TA. Conservation and divergence of plant microRNA genes. Plant J, 2006, 46(2): 243-259.
  • 9Allen E, Xie ZX, Gustafson AM, Sung GH, Spatafora JW, Carrington JC. Evolution of microRNA genes by inverted duplication of target gene sequences in Arabidopsis thaliana. Nat Genet, 2004, 36(12): 1282-1290.
  • 10Voinnet O. Origin, biogenesis, and activity of plant micro- RNAs. Cell, 2009, 136(4): 669-687.

共引文献43

同被引文献23

引证文献2

二级引证文献4

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部