摘要
植物mi RNA在调控基因表达、细胞周期、生物体发育、抗逆等方面起重要作用。为研究胡杨(Populus euphratica Oliv.)的耐盐机制,以1年生胡杨无性系幼苗为材料,构建具有空间代表性的盐胁迫胡杨c DNA文库,利用二代测序技术测定Na Cl胁迫下和正常培养条件下胡杨叶和根mi RNA表达情况。结果表明,不同的mi RNA之间表达量存在明显差异,表达丰度最高的mi RNA有mi R156、mi R157、mi R165、mi R166和mi R167等,合计占总表达量的90%以上。胡杨根部存在特异表达的mi RNA,在整个耐盐调控机制中发挥着生理调节、分子调控和信号传导等极为重要的作用。盐处理样品中发现大量响应盐胁迫的mi RNA,对这些转录因子进行靶基因预测和注释后,发现很多盐胁迫响应的mi RNA与NAC和SPL等重要转录因子家族相关,与前人的结论一致,另外还发现许多mi RNA的调控对象是ATP酶和激素响应因子。
MicroRNAs (miRNAs) have important regulatory functions in plant growth, development, cell cycle, gene expression regulation and response to abiotic stress. For studying the resistance of Populus euphratica to salt, we used 1-year-old poplar cutting clones to construct four small cDNA libraries from P. euphratica plantlets. We used the high-throughput sequencing to identify differentially expressed miRNAs treated with or without salt (300 mmol · L^-1 NaCl, 7 days). The different miRNA had significantly different expression. More than 90% expression were appeared in miR156, miR157, miR165, miR166, and miR167. The miRNA specific expressed in root played extremely important role in physiological, molecular regulation and signal transduction. Especially, many miRNAs were found in response to salt stress, and their target genes were predicted and annotated. These miRNAs were related to NAC and SPL transcription factor family, and they regulated ATP enzyme andhormone response factor.
出处
《植物研究》
CAS
CSCD
北大核心
2015年第6期836-842,共7页
Bulletin of Botanical Research
基金
国家自然科学基金(31401138)
中央高校基本科研业务费专项资金项目(TD2012-04)
关键词
高通量测序
MIRNAS
胡杨
盐胁迫
high-throughput sequencing
microRNA
Populus euphratica
salt stress